INJECTABLE COMPOSITE HYDROGEL FOR WOUND HEALING APPLICATIONS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F25%3A63595518" target="_blank" >RIV/70883521:28610/25:63595518 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

INJECTABLE COMPOSITE HYDROGEL FOR WOUND HEALING APPLICATIONS

Popis výsledku v původním jazyce

Injectable hydrogels are gaining prominence in biomedicine for applications such as tissue regeneration and wound healing [1]. Chitosan is a widely used biopolymer, but its poor solubility at physiological pH often necessitates chemical modifications that can introduce concerns about non-physiological degradation products [2].This study reports the development of novel injectable, self-healing, and anti-inflammatory composite hydrogels for tissue regeneration and wound healing, addressing the solubility limitations of chitosan. Water-soluble half-acetylated chitosan (SCN), synthesized by reacetylating chitosan to a degree of deacetylation (DD) of ~55% to ensure solubility at physiological pH without artificial functional groups, formed the hydrogel matrix. Dialdehyde cellulose (DAC), prepared via periodate oxidation of cellulose, was employed as a biocompatible crosslinker, uniquely binding SCN through dynamic Schiff base linkages and anchoring polypyrrole (PPy) nanoparticles via aldol condensation. PPy was incorporated for its established biocompatibility and therapeutic properties, including anti-inflammatory and wound healing-accelerating effects [3]. Hydrogels, also containing 5 wt% PPy colloids, were fabricated under mild conditions by mixing SCN and DAC solutions. Rheological analyses confirmed their injectable nature, characterized by shear-thinning behavior and rapid self-healing capabilities crucial for minimally invasive delivery. All formulations demonstrated excellent cytocompatibility with NIH/3T3 fibroblasts and RAW 264.7 macrophages. Notably, PPy-containing hydrogels significantly accelerated fibroblast migration in vitro, achieving up to two-fold faster wound closure compared to controls, indicating substantial wound healing promotion. Furthermore, these SCN-DAC-PPy hydrogels markedly reduced the production of pro-inflammatory mediators, nitric oxide (NO) and interleukin-6 (IL-6), by LPS-stimulated macrophages, highlighting their potent anti-inflammatory activity. Consequently, these composite hydrogels, with their combined injectability, self-healing capacity, biocompatibility, and immunomodulatory effects, hold significant promise as advanced biomaterials for wound care and tissue regeneration.

Název v anglickém jazyce

INJECTABLE COMPOSITE HYDROGEL FOR WOUND HEALING APPLICATIONS

Popis výsledku anglicky

Injectable hydrogels are gaining prominence in biomedicine for applications such as tissue regeneration and wound healing [1]. Chitosan is a widely used biopolymer, but its poor solubility at physiological pH often necessitates chemical modifications that can introduce concerns about non-physiological degradation products [2].This study reports the development of novel injectable, self-healing, and anti-inflammatory composite hydrogels for tissue regeneration and wound healing, addressing the solubility limitations of chitosan. Water-soluble half-acetylated chitosan (SCN), synthesized by reacetylating chitosan to a degree of deacetylation (DD) of ~55% to ensure solubility at physiological pH without artificial functional groups, formed the hydrogel matrix. Dialdehyde cellulose (DAC), prepared via periodate oxidation of cellulose, was employed as a biocompatible crosslinker, uniquely binding SCN through dynamic Schiff base linkages and anchoring polypyrrole (PPy) nanoparticles via aldol condensation. PPy was incorporated for its established biocompatibility and therapeutic properties, including anti-inflammatory and wound healing-accelerating effects [3]. Hydrogels, also containing 5 wt% PPy colloids, were fabricated under mild conditions by mixing SCN and DAC solutions. Rheological analyses confirmed their injectable nature, characterized by shear-thinning behavior and rapid self-healing capabilities crucial for minimally invasive delivery. All formulations demonstrated excellent cytocompatibility with NIH/3T3 fibroblasts and RAW 264.7 macrophages. Notably, PPy-containing hydrogels significantly accelerated fibroblast migration in vitro, achieving up to two-fold faster wound closure compared to controls, indicating substantial wound healing promotion. Furthermore, these SCN-DAC-PPy hydrogels markedly reduced the production of pro-inflammatory mediators, nitric oxide (NO) and interleukin-6 (IL-6), by LPS-stimulated macrophages, highlighting their potent anti-inflammatory activity. Consequently, these composite hydrogels, with their combined injectability, self-healing capacity, biocompatibility, and immunomodulatory effects, hold significant promise as advanced biomaterials for wound care and tissue regeneration.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

<a href="/cs/project/GA24-11534S" target="_blank" >GA24-11534S: Vodivé (bio)polymerní kompozity s kovalentně vázaným polypyrrolem pro biomedicínské aplikace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů