Characterization of keratin hydrolyzates prepared from sheep wool

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F14%3A43871809" target="_blank" >RIV/70883521:28610/14:43871809 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/14:43871809

Výsledek na webu

<a href="http://dx.doi.org/10.14233/ajchem.2014.16480" target="_blank" >http://dx.doi.org/10.14233/ajchem.2014.16480</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14233/ajchem.2014.16480" target="_blank" >10.14233/ajchem.2014.16480</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of keratin hydrolyzates prepared from sheep wool

Popis výsledku v původním jazyce

Preparation of keratin hydrolyzates through alkaline-enzymatic hydrolysis was already described earlier. Employment of hydrolyzates in practice requires knowledge of their composition, thermal stability and molecular weight. The aim of this contributionis to determine molecular weight of keratin hydrolyzates (prepared from sheep wool). Molecular weight (Mw) of keratin hydrolyzates was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Selection of hydrolysis conditionsallows to prepare hydrolyzates with predominant share of low-molecular fractions, intermediate-molecular fractions or high-molecular fractions. Optimal conditions for preparing keratin hydrolyzates are as follows: in 1ststage of hydrolysis, acting on wool with 0.6% KOH at 90 °C for 48 h; in 2ndstage of hydrolysis, adding 1% (w/w) proteolytic enzyme Savinase 6 T and stirring at 40°C for 24 h. Under these conditions, breakdown efficiency is 63% and a hydrolyzate is prepared containing 11.

Název v anglickém jazyce

Characterization of keratin hydrolyzates prepared from sheep wool

Popis výsledku anglicky

Preparation of keratin hydrolyzates through alkaline-enzymatic hydrolysis was already described earlier. Employment of hydrolyzates in practice requires knowledge of their composition, thermal stability and molecular weight. The aim of this contributionis to determine molecular weight of keratin hydrolyzates (prepared from sheep wool). Molecular weight (Mw) of keratin hydrolyzates was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Selection of hydrolysis conditionsallows to prepare hydrolyzates with predominant share of low-molecular fractions, intermediate-molecular fractions or high-molecular fractions. Optimal conditions for preparing keratin hydrolyzates are as follows: in 1ststage of hydrolysis, acting on wool with 0.6% KOH at 90 °C for 48 h; in 2ndstage of hydrolysis, adding 1% (w/w) proteolytic enzyme Savinase 6 T and stirring at 40°C for 24 h. Under these conditions, breakdown efficiency is 63% and a hydrolyzate is prepared containing 11.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0111" target="_blank" >ED2.1.00/03.0111: Centrum polymerních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Asian Journal of Chemistry

ISSN

0970-7077

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

5

Strana od-do

6523-6527

Kód UT WoS článku

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EID výsledku v databázi Scopus

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