Folic acid-grafted-chitosan-alginate nanocomplexes for multiple delivery of chemotherapeutic agents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F19%3A63524214" target="_blank" >RIV/70883521:28610/19:63524214 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Folic acid-grafted-chitosan-alginate nanocomplexes for multiple delivery of chemotherapeutic agents

Popis výsledku v původním jazyce

A significant challenge faced by researchers involved in the sphere of drug delivery is the development of innovative multidrug delivery systems. Herein, we focused on developing nanocomplexes based on chitosan and alginic acid suitable to allocate a combination of chemotherapeutic drugs to improve their ecacy and reduce the dosage. Conjugation with folic acid was performed to enhance the targeting. The carriers exhibited a spherical shape with a diameter in the range 70–120 nm, a ⇣-potential between 30 and 35 mV with excellent stability in human serum, and low hemolytic activity of up to 100 µg/mL. Over 800 µg of drugs per mg of the carrier were loaded and released, displaying a pH-dependent trend with no physical, chemical and biological interferences, which benefited from the advantage of having full control over the given release of the drug. In vitro studies performed on human epithelial cervix carcinoma cells and mouse fibroblast cells demonstrated that said dual-loaded complexes showed higher cytotoxicity than single-loaded and free-drug formulations. The viability of the cells decreased, thereby confirming the primary role played by the targeting molecule

Název v anglickém jazyce

Folic acid-grafted-chitosan-alginate nanocomplexes for multiple delivery of chemotherapeutic agents

Popis výsledku anglicky

A significant challenge faced by researchers involved in the sphere of drug delivery is the development of innovative multidrug delivery systems. Herein, we focused on developing nanocomplexes based on chitosan and alginic acid suitable to allocate a combination of chemotherapeutic drugs to improve their ecacy and reduce the dosage. Conjugation with folic acid was performed to enhance the targeting. The carriers exhibited a spherical shape with a diameter in the range 70–120 nm, a ⇣-potential between 30 and 35 mV with excellent stability in human serum, and low hemolytic activity of up to 100 µg/mL. Over 800 µg of drugs per mg of the carrier were loaded and released, displaying a pH-dependent trend with no physical, chemical and biological interferences, which benefited from the advantage of having full control over the given release of the drug. In vitro studies performed on human epithelial cervix carcinoma cells and mouse fibroblast cells demonstrated that said dual-loaded complexes showed higher cytotoxicity than single-loaded and free-drug formulations. The viability of the cells decreased, thereby confirming the primary role played by the targeting molecule

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

20901 - Industrial biotechnology

Projekt

<a href="/cs/project/LO1504" target="_blank" >LO1504: Centrum polymerních systémů plus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů