Morphology of Nickel-Chitosan nanoparticles

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Morphology of Nickel-Chitosan nanoparticles

Popis výsledku v původním jazyce

Chitosan is a polysaccharide and derivate of chitin. From a chemical viewpoint, is a linear copolymer composed of randomly distributed β(1-4) N-acetyl glucosamine. It dissolves only under acidic conditions because of its pKa value (6.5). It is widely used in pharmaceutical and biomedical applications because of its unique properties, such as biocompatibility, biodegradability, antibacterial activity, and non-toxicity. Chitosan nanoparticles (CSNPs) can be prepared by various techniques such as emulsion cross linking, reverse micellar method, linking spray drying, and ion-gelation method. Ion-gelation method is based on electrostatic interactions between positive and negative charges. Chitosan is a cationic polymer that can stabilize anionic substances under acidic conditions. In this case, the negative charge of polyanion - sodium tripolyphosphate (TPP) was used. TPP was continuously dropped to the chitosan solution (pH = 4.8) and stirred for 30 minutes. Thereafter, two opposite charges will combine based on physical crosslinking to chitosan nanocarriers. Prepared chitosan nanoparticles were modified with solution of 0.06 M NiCl2 . 6 H2O. Nickel solution (200 µL) was slowly dropped into the colloidal system of chitosan nanoparticles. The prepared colloidal system was centrifugated at 10 000 rpm for 15 minutes. Chitosan nanoparticles modified with nickel (Ni-CSNPs) have potential applications in electrochemical insulin sensors. Based on previous research, nickel or nickel oxide nanoparticles could be considered great candidates for electrochemical insulin sensors. Nickel shows catalytic activity at alkaline pH by creating the specific electrocatalytic anion NiOOH-. The presence of NiOOH − anions is essential for direct and rapid electrochemical insulin oxidation. The morphology of the prepared Ni-CSNPs was studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the obtained results, spherical structures were observed with the average size of 158 nm calculated from the TEM image. Size of CSNPs based on TEM images is shown in histogram. TEM image shows black dost, which indicates that nickel have been attached to the surface of chitosan nanoparticles. The EDX analysis obtained from the red area of the SEM image indicated 7.24 wt. % nickel, 29.1 wt% of carbon, 36.3 wt.% of oxygen and less than 1 wt.% of chlorine. The presence of chlorine is caused by the addition of nickel chloride to the already-formed chitosan nanoparticles. Based on results of the microscopic methods and EDX analysis, the surfaces of the chitosan nanoparticles were successfully modified by nickel. Electrodes modified with Ni-CSNPs show potential as electrochemical sensors for direct and fast insulin oxidation. The development of insulin electrochemical sensors is necessary because of the worldwide increase in the number of people suffering from diabetes mellitus.

Název v anglickém jazyce

Morphology of Nickel-Chitosan nanoparticles

Popis výsledku anglicky

Chitosan is a polysaccharide and derivate of chitin. From a chemical viewpoint, is a linear copolymer composed of randomly distributed β(1-4) N-acetyl glucosamine. It dissolves only under acidic conditions because of its pKa value (6.5). It is widely used in pharmaceutical and biomedical applications because of its unique properties, such as biocompatibility, biodegradability, antibacterial activity, and non-toxicity. Chitosan nanoparticles (CSNPs) can be prepared by various techniques such as emulsion cross linking, reverse micellar method, linking spray drying, and ion-gelation method. Ion-gelation method is based on electrostatic interactions between positive and negative charges. Chitosan is a cationic polymer that can stabilize anionic substances under acidic conditions. In this case, the negative charge of polyanion - sodium tripolyphosphate (TPP) was used. TPP was continuously dropped to the chitosan solution (pH = 4.8) and stirred for 30 minutes. Thereafter, two opposite charges will combine based on physical crosslinking to chitosan nanocarriers. Prepared chitosan nanoparticles were modified with solution of 0.06 M NiCl2 . 6 H2O. Nickel solution (200 µL) was slowly dropped into the colloidal system of chitosan nanoparticles. The prepared colloidal system was centrifugated at 10 000 rpm for 15 minutes. Chitosan nanoparticles modified with nickel (Ni-CSNPs) have potential applications in electrochemical insulin sensors. Based on previous research, nickel or nickel oxide nanoparticles could be considered great candidates for electrochemical insulin sensors. Nickel shows catalytic activity at alkaline pH by creating the specific electrocatalytic anion NiOOH-. The presence of NiOOH − anions is essential for direct and rapid electrochemical insulin oxidation. The morphology of the prepared Ni-CSNPs was studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the obtained results, spherical structures were observed with the average size of 158 nm calculated from the TEM image. Size of CSNPs based on TEM images is shown in histogram. TEM image shows black dost, which indicates that nickel have been attached to the surface of chitosan nanoparticles. The EDX analysis obtained from the red area of the SEM image indicated 7.24 wt. % nickel, 29.1 wt% of carbon, 36.3 wt.% of oxygen and less than 1 wt.% of chlorine. The presence of chlorine is caused by the addition of nickel chloride to the already-formed chitosan nanoparticles. Based on results of the microscopic methods and EDX analysis, the surfaces of the chitosan nanoparticles were successfully modified by nickel. Electrodes modified with Ni-CSNPs show potential as electrochemical sensors for direct and fast insulin oxidation. The development of insulin electrochemical sensors is necessary because of the worldwide increase in the number of people suffering from diabetes mellitus.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů