Dissemination of the bla(CTX-M-15) gene among Enterobacteriaceae via outer membrane vesicles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F20%3A00013248" target="_blank" >RIV/75010330:_____/20:00013248 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/jac/article/75/9/2442/5860391" target="_blank" >https://academic.oup.com/jac/article/75/9/2442/5860391</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jac/dkaa214" target="_blank" >10.1093/jac/dkaa214</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dissemination of the bla(CTX-M-15) gene among Enterobacteriaceae via outer membrane vesicles

Popis výsledku v původním jazyce

Background: Bacterial outer membrane vesicles (OMVs) are an emerging source of antibiotic resistance transfer but their role in the spread of the bla(CTX-M-15) gene encoding the most frequent CTX-M ESBL in Enterobacteriaceae is unknown. Objectives: To determine the presence of bla(CTX-M-15) and other antibiotic resistance genes in OMVs of the CTX-M-15-producing MDR Escherichia coli O104:H4 outbreak strain and the ability of these OMVs to spread these genes among Enterobacteriaceae under different conditions. Methods: OMV-borne antibiotic resistance genes were detected by PCR. OMV-mediated transfer of bla(CTX-M-15) and the associated bla(TEM-1) was quantified under laboratory conditions, simulated intraintestinal conditions and under ciprofloxacin stress; resistance to antibiotics and the ESBL phenotype were determined by the CLSI disc diffusion methods and the presence of pESBL by plasmid profiling and Southern blot hybridization. Results: E. coli O104:H4 OMVs carried bla(CTX-M-15) and bla(TEM-1) located on the pESBL plasmid, but not chromosomal antibiotic resistance genes. The OMVs transferred bla(CTX-M-15), bla(TEM-1) and the associated pESBL into Enterobacteriaceae of different species. The frequencies of the OMV-mediated transfer were significantly increased under simulated intraintestinal conditions and under ciprofloxacin stress when compared with laboratory conditions. The 'vesiculants' (i.e. recipients that received the bla(CTX-M-15)-and bla(TEM-1)-harbouring pESBL via OMVs) acquired resistance to cefotaxime, ceftazidime and cefpodoxime and expressed the ESBL phenotype. They were able to further spread pESBL and the bla(CTX-M-15) and bla(TEM-1) genes via OMVs.

Název v anglickém jazyce

Dissemination of the bla(CTX-M-15) gene among Enterobacteriaceae via outer membrane vesicles

Popis výsledku anglicky

Background: Bacterial outer membrane vesicles (OMVs) are an emerging source of antibiotic resistance transfer but their role in the spread of the bla(CTX-M-15) gene encoding the most frequent CTX-M ESBL in Enterobacteriaceae is unknown. Objectives: To determine the presence of bla(CTX-M-15) and other antibiotic resistance genes in OMVs of the CTX-M-15-producing MDR Escherichia coli O104:H4 outbreak strain and the ability of these OMVs to spread these genes among Enterobacteriaceae under different conditions. Methods: OMV-borne antibiotic resistance genes were detected by PCR. OMV-mediated transfer of bla(CTX-M-15) and the associated bla(TEM-1) was quantified under laboratory conditions, simulated intraintestinal conditions and under ciprofloxacin stress; resistance to antibiotics and the ESBL phenotype were determined by the CLSI disc diffusion methods and the presence of pESBL by plasmid profiling and Southern blot hybridization. Results: E. coli O104:H4 OMVs carried bla(CTX-M-15) and bla(TEM-1) located on the pESBL plasmid, but not chromosomal antibiotic resistance genes. The OMVs transferred bla(CTX-M-15), bla(TEM-1) and the associated pESBL into Enterobacteriaceae of different species. The frequencies of the OMV-mediated transfer were significantly increased under simulated intraintestinal conditions and under ciprofloxacin stress when compared with laboratory conditions. The 'vesiculants' (i.e. recipients that received the bla(CTX-M-15)-and bla(TEM-1)-harbouring pESBL via OMVs) acquired resistance to cefotaxime, ceftazidime and cefpodoxime and expressed the ESBL phenotype. They were able to further spread pESBL and the bla(CTX-M-15) and bla(TEM-1) genes via OMVs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30303 - Infectious Diseases

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Antimicrobial Chemotherapy

ISSN

0305-7453

e-ISSN

1460-2091

Svazek periodika

75

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

2442-2451

Kód UT WoS článku

000574367100010

EID výsledku v databázi Scopus

2-s2.0-85089807470