Exprese a lokalizace inhibitoru akrosinu v kančím reprodukčním traktu
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F08%3A00323277" target="_blank" >RIV/86652036:_____/08:00323277 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Expression and localization of acrosin inhibitor in the boar reproductive tract
Popis výsledku v původním jazyce
The main role of proteinase inhibitors in the reproductive tract is to inactivate prematurely released hydrolytic enzymes from damaged spermatozoa and protect the male and female reproductive tracts and spermatozoa against proteolytic degradation. We studied the expression and localization of acrosin inhibitor isolated from seminal plasma and spermatozoa in the boar reproductive tract. The amino acid sequence of boar acrosin inhibitor and its biochemical properties were completely determined. However, its origin and localization have not been fully elucidated. Acrosin inhibitor mRNA was detectable in epididymis, seminal vesicles, prostate and Cowper?s glands. Polyclonal antibody against purified acrosin inhibitor detected and localized this protein inextracts and secretory tissues of boar reproductive organs. The acrosin inhibitor was immunodetected in epididymis, prostate and seminal vesicles, and additionally in sperm extracts and on epididymal and ejaculated spermatozoa.
Název v anglickém jazyce
Expression and localization of acrosin inhibitor in the boar reproductive tract
Popis výsledku anglicky
The main role of proteinase inhibitors in the reproductive tract is to inactivate prematurely released hydrolytic enzymes from damaged spermatozoa and protect the male and female reproductive tracts and spermatozoa against proteolytic degradation. We studied the expression and localization of acrosin inhibitor isolated from seminal plasma and spermatozoa in the boar reproductive tract. The amino acid sequence of boar acrosin inhibitor and its biochemical properties were completely determined. However, its origin and localization have not been fully elucidated. Acrosin inhibitor mRNA was detectable in epididymis, seminal vesicles, prostate and Cowper?s glands. Polyclonal antibody against purified acrosin inhibitor detected and localized this protein inextracts and secretory tissues of boar reproductive organs. The acrosin inhibitor was immunodetected in epididymis, prostate and seminal vesicles, and additionally in sperm extracts and on epididymal and ejaculated spermatozoa.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
DN - Vliv životního prostředí na zdraví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2008
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů