Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00376560" target="_blank" >RIV/86652036:_____/12:00376560 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/12:10296031

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >http://dx.doi.org/10.1016/j.cca.2012.01.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >10.1016/j.cca.2012.01.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

Popis výsledku v původním jazyce

Background: Although important improvements of downstream molecular in vitro diagnostics assays based on RNA from blood were made, the pre-analytical workflow is still poorly defined. Methods: We performed a multicenter study within the EU-granted SPIDIAproject to investigate blood collection and shipping influence on the following RNA quality parameters: yield, purity, integrity, RT-qPCR interference and IL1B, IL8, FOS and GAPDH gene expression. Two models were designed: Exp A. Ten laboratories collected blood from an own donor into two different tubes (with or without stabilizer) and extracted RNA at two different times; Exp B. Blood was drawn from a single donor and shipped to ten laboratories in two different tubes (with or without stabilizer) forRNA extraction. Results: In both models and collection tubes, reliable results were obtained for purity, yield, GAPDH expression, and interferences. A substantial variation in RIN (Exp A) and in transcription levels of IL1B. IL8 and FOS

Název v anglickém jazyce

Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

Popis výsledku anglicky

Background: Although important improvements of downstream molecular in vitro diagnostics assays based on RNA from blood were made, the pre-analytical workflow is still poorly defined. Methods: We performed a multicenter study within the EU-granted SPIDIAproject to investigate blood collection and shipping influence on the following RNA quality parameters: yield, purity, integrity, RT-qPCR interference and IL1B, IL8, FOS and GAPDH gene expression. Two models were designed: Exp A. Ten laboratories collected blood from an own donor into two different tubes (with or without stabilizer) and extracted RNA at two different times; Exp B. Blood was drawn from a single donor and shipped to ten laboratories in two different tubes (with or without stabilizer) forRNA extraction. Results: In both models and collection tubes, reliable results were obtained for purity, yield, GAPDH expression, and interferences. A substantial variation in RIN (Exp A) and in transcription levels of IL1B. IL8 and FOS

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinica Chimica Acta

ISSN

0009-8981

e-ISSN

—

Svazek periodika

413

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

779-786

Kód UT WoS článku

000301908000023

EID výsledku v databázi Scopus

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