Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00442689" target="_blank" >RIV/86652036:_____/14:00442689 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1371/journal.pone.0111644" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0111644</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0111644" target="_blank" >10.1371/journal.pone.0111644</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples
Popis výsledku v původním jazyce
There is an increasing need for proper quality control tools in the pre-analytical phase of the molecular diagnostic workflow. The aim of the present study was to identify biomarkers for monitoring pre-analytical mRNA quality variations in two differenttypes of blood collection tubes, K(2)EDTA (EDTA) tubes and PAXgene Blood RNA Tubes (PAXgene tubes). These tubes are extensively used both in the diagnostic setting as well as for research biobank samples. Blood specimens collected in the two different blood collection tubes were stored for varying times at different temperatures, and microarray analysis was performed on resultant extracted RNA. A large set of potential mRNA quality biomarkers for monitoring post-phlebotomy gene expression changes and mRNA degradation in blood was identified. qPCR assays for the potential biomarkers and a set of relevant reference genes were generated and used to pre-validate a sub-set of the selected biomarkers. The assay precision of the potential qPCR
Název v anglickém jazyce
Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples
Popis výsledku anglicky
There is an increasing need for proper quality control tools in the pre-analytical phase of the molecular diagnostic workflow. The aim of the present study was to identify biomarkers for monitoring pre-analytical mRNA quality variations in two differenttypes of blood collection tubes, K(2)EDTA (EDTA) tubes and PAXgene Blood RNA Tubes (PAXgene tubes). These tubes are extensively used both in the diagnostic setting as well as for research biobank samples. Blood specimens collected in the two different blood collection tubes were stored for varying times at different temperatures, and microarray analysis was performed on resultant extracted RNA. A large set of potential mRNA quality biomarkers for monitoring post-phlebotomy gene expression changes and mRNA degradation in blood was identified. qPCR assays for the potential biomarkers and a set of relevant reference genes were generated and used to pre-validate a sub-set of the selected biomarkers. The assay precision of the potential qPCR
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS ONE
ISSN
1932-6203
e-ISSN
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Svazek periodika
9
Číslo periodika v rámci svazku
11/e111644
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
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Kód UT WoS článku
000344402000081
EID výsledku v databázi Scopus
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