Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00379059" target="_blank" >RIV/86652036:_____/12:00379059 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009" target="_blank" >http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009" target="_blank" >10.1016/j.clinbiochem.2012.02.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Popis výsledku v původním jazyce

Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects

Název v anglickém jazyce

Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Popis výsledku anglicky

Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F08%2F0811" target="_blank" >GA204/08/0811: Mechanismus tvorby kyslíkových radikálů a mitochondriální permeabilizace</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Biochemistry

ISSN

0009-9120

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

575-581

Kód UT WoS článku

000303553300013

EID výsledku v databázi Scopus

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