Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F17%3A00475858" target="_blank" >RIV/86652036:_____/17:00475858 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10363378

Výsledek na webu

<a href="http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011" target="_blank" >http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011" target="_blank" >dx.doi.org/10.1016/j.theriogenology.2017.06.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

Popis výsledku v původním jazyce

Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (a-L-fucosidase, a-D-galactosidase, b-D-galactosidase, b-D-N- ylhexosaminidase, and a-Dmannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of a-D-mannosidase, which was increased (P < 0.001), and b-D-galactosidase, which was decreased (P < 0.001) at neutral pH, and of a-D-galactosidase and b-N-acetylhexosaminidase, which were decreased (P < 0.0001) at the acidic pH. The comparison of glycosidases from follicular fluid and from blood plasma using red native electrophoresis revealed that most of the glycosidases are present in more than one isoenzyme form, some of them were detected mainly in the follicular fluid. Finally, we tested the effect of glycosidases on the interaction between zona pellucida and AWN 1 spermadhesin (putative sperm receptor of zona pellucida) and demonstrated that the effect of both b-D-galactosidase and to a lesser degree a-D-mannosidase led to a decrease in this interaction. We can hypothesize that these two glycosidases modulate the amount of zona pellucida oligosaccharide moieties and/or their structures for an optimal sperm binding in pigs.

Název v anglickém jazyce

Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

Popis výsledku anglicky

Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (a-L-fucosidase, a-D-galactosidase, b-D-galactosidase, b-D-N- ylhexosaminidase, and a-Dmannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of a-D-mannosidase, which was increased (P < 0.001), and b-D-galactosidase, which was decreased (P < 0.001) at neutral pH, and of a-D-galactosidase and b-N-acetylhexosaminidase, which were decreased (P < 0.0001) at the acidic pH. The comparison of glycosidases from follicular fluid and from blood plasma using red native electrophoresis revealed that most of the glycosidases are present in more than one isoenzyme form, some of them were detected mainly in the follicular fluid. Finally, we tested the effect of glycosidases on the interaction between zona pellucida and AWN 1 spermadhesin (putative sperm receptor of zona pellucida) and demonstrated that the effect of both b-D-galactosidase and to a lesser degree a-D-mannosidase led to a decrease in this interaction. We can hypothesize that these two glycosidases modulate the amount of zona pellucida oligosaccharide moieties and/or their structures for an optimal sperm binding in pigs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theriogenelogy

ISSN

0093-691X

e-ISSN

—

Svazek periodika

100

Číslo periodika v rámci svazku

Sep 15 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

80-87

Kód UT WoS článku

000406985600011

EID výsledku v databázi Scopus

2-s2.0-85020545089