Modification of the zona pellucida sperm-binding protein 3 (ZP3) in pigs during folliculogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908809" target="_blank" >RIV/62156489:43210/14:43908809 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Modification of the zona pellucida sperm-binding protein 3 (ZP3) in pigs during folliculogenesis

Popis výsledku v původním jazyce

Polyspermic fertilization is still one of major problems during in vitro fertilization in pigs. The zona pellucida is an extracellular matrix surrounding mammalian oocytes, plays an important role during fertilization and forms polyspermy block after fertilization as well. The porcine zona is composed of three glycoprotein called ZP2, ZP3 and ZP4. The purpose of this study is to evaluate modifications of the zona pellucida sperm-binding protein 3 (ZP3) of oocytes with different developmental competence.This protein is involved in binding of the spermatozoa to the oocyte. Using western blot analysis, it was found no significant differences in expression of ZP3 protein in oocytes with lower developmental competence (from small follicles), in compared tooocytes with higher developmental competence (from medium follicles). But it was detected band in area >50 kDa, which could play an essential role during fertilization of oocytes.

Název v anglickém jazyce

Modification of the zona pellucida sperm-binding protein 3 (ZP3) in pigs during folliculogenesis

Popis výsledku anglicky

Polyspermic fertilization is still one of major problems during in vitro fertilization in pigs. The zona pellucida is an extracellular matrix surrounding mammalian oocytes, plays an important role during fertilization and forms polyspermy block after fertilization as well. The porcine zona is composed of three glycoprotein called ZP2, ZP3 and ZP4. The purpose of this study is to evaluate modifications of the zona pellucida sperm-binding protein 3 (ZP3) of oocytes with different developmental competence.This protein is involved in binding of the spermatozoa to the oocyte. Using western blot analysis, it was found no significant differences in expression of ZP3 protein in oocytes with lower developmental competence (from small follicles), in compared tooocytes with higher developmental competence (from medium follicles). But it was detected band in area >50 kDa, which could play an essential role during fertilization of oocytes.

Druh

D - Stať ve sborníku

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2014: Proceedings of International Ph.D. Students Conference

ISBN

978-80-7509-174-1

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

212-215

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

19. 11. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000362518300042