Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F22%3A00556615" target="_blank" >RIV/86652036:_____/22:00556615 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/22:43904435

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0044848622000138?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0044848622000138?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2022.737899" target="_blank" >10.1016/j.aquaculture.2022.737899</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage

Popis výsledku v původním jazyce

Generally, holoblastic cleavage in embryos (as in amphibians) in which all blastomeres contribute to one of the germ layers, are preserved as a stem lineage of vertebrates, and meroblastic cleavage has evolved independently in each vertebrate lineage. The increasing egg size: yolk volume is the key factor for transition from holoblastic to meroblastic cleavage patterns. Sturgeon (Acipenser) eggs are two times larger than those of the African clawed frog Xenopus laevis (amphibian), despite the varying size, sturgeon embryos retain nearly the same developmental characteristics as X. laevis. Comparatively, the fate of blastomeres derived from the vegetal pole (VP) of a sturgeon embryo is unspecified. Thus, the goal of this study was to determine whether the VP of the embryo contributes to embryonic development, or is simply extra-embryonic. This may also reveal whether the transition of the cleavage pattern (holoblastic to meroblastic) in the actinopterygian lineage is correlated with the egg size: yolk volume. Here, we found that sturgeon vegetal blastomeres formed only primordial germ cells, and the rest were made up of cellular yolk (yolk cells, YCs). Morphological and phenotypic characteristics revealed that after the 1 k-cell / mid-blastula transition, YCs became transcriptionally inactive and served only to provide nutrition to larvae as they developed. Furthermore, inhibition of vegetal blastomeres revealed that sturgeon can utilize their yolk in an acellular form, similar to teleosts, implying that meroblastic cleavage in the actinopterygians, like teleosts, might have evolved by the fusion of the vegetal blastomeres.

Název v anglickém jazyce

Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage

Popis výsledku anglicky

Generally, holoblastic cleavage in embryos (as in amphibians) in which all blastomeres contribute to one of the germ layers, are preserved as a stem lineage of vertebrates, and meroblastic cleavage has evolved independently in each vertebrate lineage. The increasing egg size: yolk volume is the key factor for transition from holoblastic to meroblastic cleavage patterns. Sturgeon (Acipenser) eggs are two times larger than those of the African clawed frog Xenopus laevis (amphibian), despite the varying size, sturgeon embryos retain nearly the same developmental characteristics as X. laevis. Comparatively, the fate of blastomeres derived from the vegetal pole (VP) of a sturgeon embryo is unspecified. Thus, the goal of this study was to determine whether the VP of the embryo contributes to embryonic development, or is simply extra-embryonic. This may also reveal whether the transition of the cleavage pattern (holoblastic to meroblastic) in the actinopterygian lineage is correlated with the egg size: yolk volume. Here, we found that sturgeon vegetal blastomeres formed only primordial germ cells, and the rest were made up of cellular yolk (yolk cells, YCs). Morphological and phenotypic characteristics revealed that after the 1 k-cell / mid-blastula transition, YCs became transcriptionally inactive and served only to provide nutrition to larvae as they developed. Furthermore, inhibition of vegetal blastomeres revealed that sturgeon can utilize their yolk in an acellular form, similar to teleosts, implying that meroblastic cleavage in the actinopterygians, like teleosts, might have evolved by the fusion of the vegetal blastomeres.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

551

Číslo periodika v rámci svazku

MAR 30 2022

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

737899

Kód UT WoS článku

000773652600004

EID výsledku v databázi Scopus

2-s2.0-85123284517