Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902503" target="_blank" >RIV/60076658:12520/21:43902503 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/21:00550588

Výsledek na webu

<a href="https://doi.org/10.1007/s12562-020-01481-7" target="_blank" >https://doi.org/10.1007/s12562-020-01481-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12562-020-01481-7" target="_blank" >10.1007/s12562-020-01481-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo

Popis výsledku v původním jazyce

The cleavage pattern of a vertebrate&apos;s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde (PUA), 2,4-decadienal (DD)-a model aldehyde for experimental studies-adversely affects the developing embryos of several aquatic species. In this study, we employed DD for inhibition of cleavage of a definite blastomere in sturgeon embryos under various conditions. The effective treatment was found to be a combination of DD injection (0.01 v/v) and visible light (44.86-91.15 W m(-2)). Notably, DD injection or light irradiation alone cannot inhibit cleavage. Furthermore, spatial RNA localization analysis using quantitative polymerase chain reaction (qPCR)-tomography revealed that the localized pattern of selected maternal messenger ribonucleic acids (mRNAs) remained constant along the animal-vegetal (A-V) axis, which suggests that RNA localization is completed by the end of oogenesis and that early embryonic cleavage is not required for A-V asymmetry preservation.

Název v anglickém jazyce

Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo

Popis výsledku anglicky

The cleavage pattern of a vertebrate&apos;s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde (PUA), 2,4-decadienal (DD)-a model aldehyde for experimental studies-adversely affects the developing embryos of several aquatic species. In this study, we employed DD for inhibition of cleavage of a definite blastomere in sturgeon embryos under various conditions. The effective treatment was found to be a combination of DD injection (0.01 v/v) and visible light (44.86-91.15 W m(-2)). Notably, DD injection or light irradiation alone cannot inhibit cleavage. Furthermore, spatial RNA localization analysis using quantitative polymerase chain reaction (qPCR)-tomography revealed that the localized pattern of selected maternal messenger ribonucleic acids (mRNAs) remained constant along the animal-vegetal (A-V) axis, which suggests that RNA localization is completed by the end of oogenesis and that early embryonic cleavage is not required for A-V asymmetry preservation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fisheries Science

ISSN

0919-9268

e-ISSN

1444-2906

Svazek periodika

87

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

13

Strana od-do

71-83

Kód UT WoS článku

000604838000001

EID výsledku v databázi Scopus

2-s2.0-85098769135