RNA localization during early development of the axolotl

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00583145" target="_blank" >RIV/86652036:_____/23:00583145 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10472359

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fcell.2023.1260795/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2023.1260795/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcell.2023.1260795" target="_blank" >10.3389/fcell.2023.1260795</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RNA localization during early development of the axolotl

Popis výsledku v původním jazyce

The asymmetric localization of biomolecules is critical for body plan development. One of the most popular model organisms for early embryogenesis studies is Xenopus laevis but there is a lack of information in other animal species. Here, we compared the early development of two amphibian species-the frog X. laevis and the axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along the animal-vegetal axis during the early development of A. mexicanum. For that purpose, we performed spatial transcriptome-wide analysis at low resolution, which revealed dynamic changes along the animal-vegetal axis classified into the following categories: profile alteration, de novo synthesis and degradation. Surprisingly, our results showed that many of the vegetally localized genes, which are important for germ cell development, are degraded during early development. Furthermore, we assessed the motif presence in UTRs of degraded mRNAs and revealed the enrichment of several motifs in RNAs of germ cell markers. Our results suggest novel reorganization of the transcriptome during embryogenesis of A. mexicanum to converge to the similar developmental pattern as the X. laevis.

Název v anglickém jazyce

RNA localization during early development of the axolotl

Popis výsledku anglicky

The asymmetric localization of biomolecules is critical for body plan development. One of the most popular model organisms for early embryogenesis studies is Xenopus laevis but there is a lack of information in other animal species. Here, we compared the early development of two amphibian species-the frog X. laevis and the axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along the animal-vegetal axis during the early development of A. mexicanum. For that purpose, we performed spatial transcriptome-wide analysis at low resolution, which revealed dynamic changes along the animal-vegetal axis classified into the following categories: profile alteration, de novo synthesis and degradation. Surprisingly, our results showed that many of the vegetally localized genes, which are important for germ cell development, are degraded during early development. Furthermore, we assessed the motif presence in UTRs of degraded mRNAs and revealed the enrichment of several motifs in RNAs of germ cell markers. Our results suggest novel reorganization of the transcriptome during embryogenesis of A. mexicanum to converge to the similar developmental pattern as the X. laevis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/GA20-23836S" target="_blank" >GA20-23836S: Jeseter jako unikátní model evolučního přechodu z holoblastického na meroblastické rýhování a vývoje endodermu obratlovců</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Cell and Developmental Biology

ISSN

2296-634X

e-ISSN

2296-634X

Svazek periodika

11

Číslo periodika v rámci svazku

OCT 19 2023

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

1260795

Kód UT WoS článku

001095794600001

EID výsledku v databázi Scopus

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