Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F22%3A00559401" target="_blank" >RIV/86652036:_____/22:00559401 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/22:43904521 RIV/00216208:11310/22:10448332

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0012160622001300?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0012160622001300?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ydbio.2022.06.013" target="_blank" >10.1016/j.ydbio.2022.06.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq

Popis výsledku v původním jazyce

Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and establishment of many biological processes, cell types and the body plan. However, our knowledge about evolutionary conservation of localized transcripts is still limited to a few models. Our goal was to compare localization profiles along the animal-vegetal axis of mature eggs from four vertebrate models, two amphibians (Xenopus laevis, Ambystoma mexicanum) and two fishes (Acipenser ruthenus, Danio rerio) using the spatial expression method called TOMO-Seq. We revealed that RNAs of many known important transcripts such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models. It was also observed that there was a poor correlation between the vegetally localized transcripts but a relatively good correlation between the animally localized transcripts. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model.

Název v anglickém jazyce

Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq

Popis výsledku anglicky

Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and establishment of many biological processes, cell types and the body plan. However, our knowledge about evolutionary conservation of localized transcripts is still limited to a few models. Our goal was to compare localization profiles along the animal-vegetal axis of mature eggs from four vertebrate models, two amphibians (Xenopus laevis, Ambystoma mexicanum) and two fishes (Acipenser ruthenus, Danio rerio) using the spatial expression method called TOMO-Seq. We revealed that RNAs of many known important transcripts such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models. It was also observed that there was a poor correlation between the vegetally localized transcripts but a relatively good correlation between the animally localized transcripts. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Biology

ISSN

0012-1606

e-ISSN

1095-564X

Svazek periodika

489

Číslo periodika v rámci svazku

SEP 2022

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

146-160

Kód UT WoS článku

000823105200005

EID výsledku v databázi Scopus

2-s2.0-85133191160