Physiological phenotyping of mammalian cell lines by enzymatic activity fngerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00510085" target="_blank" >RIV/86652079:_____/19:00510085 - isvavai.cz</a>

Výsledek na webu

<a href="https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-019-4697-y" target="_blank" >https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-019-4697-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13104-019-4697-y" target="_blank" >10.1186/s13104-019-4697-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physiological phenotyping of mammalian cell lines by enzymatic activity fngerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Popis výsledku v původním jazyce

Objective Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze–thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.

Název v anglickém jazyce

Physiological phenotyping of mammalian cell lines by enzymatic activity fngerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Popis výsledku anglicky

Objective Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze–thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 - Rozvoj Centra pro studium dopadů globální změny klimatu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Research Notes

ISSN

1756-0500

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

OCT 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

682

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85073747408