Simple and semi-high throughput determination of total phenolic , anthocyanin, flavonoid content, and total antioxidant capacity of model and crop plants for cell physiological phenotyping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F25%3A00636671" target="_blank" >RIV/86652079:_____/25:00636671 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubmed.ncbi.nlm.nih.gov/40274192/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/40274192/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2025.112524" target="_blank" >10.1016/j.plantsci.2025.112524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simple and semi-high throughput determination of total phenolic , anthocyanin, flavonoid content, and total antioxidant capacity of model and crop plants for cell physiological phenotyping

Popis výsledku v původním jazyce

Plants biosynthesize a wide range of antioxidants capable of attenuating ROS-induced oxidative damage. There exist several in vitro methods to analyze antioxidants and total antioxidant capacity from different tissues and of various plant species. We have established a single, fast and cost-efficient extraction protocol combined with a semihigh throughput 96-well plate assay methods for determination of the level of the key antioxidants phenolics, anthocyanins and flavonoids in combination with the determination of total antioxidant capacity using ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). The method was optimized and verified with samples from different strawberry species and cultivars with known differences in the parameters measured. This method proved to be suitable for analyses of eight model and crop plants, and distinct antioxidant signatures were determined for the different tissues and organs analyzed, including leaf, root, fruit, spike, and tuber samples. The method was robust and was shown in two case studies to be a resourceefficient and fast experimental platform also to assess biotic and abiotic stress responses, notably including fungal infection and the impact of a progressive drought regime. Since method was adapted for a semi-high throughput 96-well assay format it is well-suited for integration of cell physiological phenotyping into a holistic phenomics approach for germplasm assessment and plant breeding screening. This analytical platform uses microplate spectrophotometer which proved to be suitable to determine the antioxidant contents and total antioxidant capacity signatures of various plant species and tissues with similar findings as reported in literature.

Název v anglickém jazyce

Simple and semi-high throughput determination of total phenolic , anthocyanin, flavonoid content, and total antioxidant capacity of model and crop plants for cell physiological phenotyping

Popis výsledku anglicky

Plants biosynthesize a wide range of antioxidants capable of attenuating ROS-induced oxidative damage. There exist several in vitro methods to analyze antioxidants and total antioxidant capacity from different tissues and of various plant species. We have established a single, fast and cost-efficient extraction protocol combined with a semihigh throughput 96-well plate assay methods for determination of the level of the key antioxidants phenolics, anthocyanins and flavonoids in combination with the determination of total antioxidant capacity using ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). The method was optimized and verified with samples from different strawberry species and cultivars with known differences in the parameters measured. This method proved to be suitable for analyses of eight model and crop plants, and distinct antioxidant signatures were determined for the different tissues and organs analyzed, including leaf, root, fruit, spike, and tuber samples. The method was robust and was shown in two case studies to be a resourceefficient and fast experimental platform also to assess biotic and abiotic stress responses, notably including fungal infection and the impact of a progressive drought regime. Since method was adapted for a semi-high throughput 96-well assay format it is well-suited for integration of cell physiological phenotyping into a holistic phenomics approach for germplasm assessment and plant breeding screening. This analytical platform uses microplate spectrophotometer which proved to be suitable to determine the antioxidant contents and total antioxidant capacity signatures of various plant species and tissues with similar findings as reported in literature.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/LM2023048" target="_blank" >LM2023048: Česká infrastruktura sledování uhlíku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

1873-2259

Svazek periodika

357

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

112524

Kód UT WoS článku

001486256300001

EID výsledku v databázi Scopus

2-s2.0-105004056491