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Výsledek výzkumu

Whole genome amplification induced bias in the detection of KRAS-mutated cell populations during colorectal carcinoma tissue testing

Whole genome amplification replicates the entire DNA content of a sample and can thus help to circumvent material limitations when insufficient DNA is available for planned genetic analyses....

FD - Onkologie a hematologie

  • 2015
  • Jx
  • Odkaz
Výsledek výzkumu

Whole genome amplification effect on segmental copy-number changes and copy-number neutral loss of heterozygosity analysis by oligonucleotide-array based comparative genomic hybridization in human

Whole genome amplification (WGA) is an approach designed to overcome small amounts of DNA for genome-wide genetic tests used in many clinical applications the absence of amplification bias. In this study, ...

EB - Genetika a molekulární biologie

  • 2016
  • Jx
Výsledek výzkumu

Software application for a design of universal pre-amplification primers for DNA profiling.

Before DNA profiling is performed in the condition of the lack of biological material, it is advisable to use pre-amplification.

EB - Genetika a molekulární biologie

  • 2010
  • D
Výsledek výzkumu

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

The objectives of this study were established to investigate the whole chromosome set of in vivo obtained early pig embryos, applying methods of whole genome amplification and comparative genomic hybridiza...

EB - Genetika a molekulární biologie

  • 2009
  • Jx
Výsledek výzkumu

Testing the impact of Whole Genome Amplification on genome comparison using the polyploid flagellated Giardia duodenalis as a model

that cannot be cultivated in vitro, as is the case for many parasites. Therefore, Whole Genome Amplification (WGA) of genomic DNA is used to overcome this limitationThe availability of high quality genomic

Biological sciences

  • 2019
  • Jimp
  • Odkaz
Výsledek výzkumu

Whole genome amplification effect on segmental copy-number changes and copy-number neutral loss of heterozygosity analysis by oligonucleotide-based array-comparative genomic hybridization in human

Whole genome amplification (WGA) is an approach designed to overcome small amounts of DNA for genome-wide genetic tests used in many clinical applications the absence of amplification bias. In this study, ...

FD - Onkologie a hematologie

  • 2016
  • Jx
Výsledek výzkumu

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samplesBackground. Whole-genome...

Microbiology

  • 2021
  • Jimp
  • Odkaz
Výsledek výzkumu

Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley

. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Results: Here we report a protocol optimized in barley including and chromosome arms which can be used for targeted

EB - Genetika a molekulární biologie

  • 2008
  • Jx
Výsledek výzkumu

Preimplantation Genetic Diagnostics

Preimplantation Genetic Diagnostics of Monogenic-based Diseases: Possibiliteies, Pitfalls and Fires Accomplishments in the Czech Republic.

EB - Genetika a molekulární biologie

  • 2008
  • Jx
Výsledek výzkumu

Genome position and gene amplification

Amplifications, regions of focal high-level copy number change, lead of genome position on propensity to amplify, we integrated a mutant form of the gene encoding dihydrofolate reductase into different positions in the human gen...

BO - Biofyzika

  • 2007
  • Jx
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