Multivalent glycopolymers as ligands of lectin-denoted biological targets
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
INTER-EXCELLENCE 2 (SMSM2016LTC01)
Main participants
Mikrobiologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-15304/2017-1
Alternative language
Project name in Czech
Multivalentní glykopolymery jako ligandy biologických struktur s lektinovou prezentací
Annotation in Czech
Vypracujeme přípravu nových multivalentních biokompatibilních konjugátů HPMA polymerů s různými definovanými oligosacharidovými ligandy. Sacharidové struktury budou připraveny chemoenzymovou syntézou za použití škály mutantních a nativních glykosidas a glykosyltransferas. Výsledné konjugáty budou otestovány jako ligandy pro vybrané lektiny, zvláště pro galektin-3, v biologických studiích in vitro ve vazebných testech a tkáňových kulturách. Bude vyhodnocena studie vztahu mezi strukturou konjugátů a jejich vazebnou afinitou vzhledem k povrchové hustotě sacharidových ligandů, jejich délce, způsobu připojení na polymer a typu kopolymerace polymerního nosiče. Biologické studie, strukturně vazebná analýza a posouzení využitelnosti připravených konjugátů budou realizovány ve spolupráci s partnery projektu COST a s využitím vlastní infrastruktury.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
CE - Biochemistry
CEP - secondary branch
CC - Organic chemistry
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10401 - Organic chemistry<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Specific carbohydrate ligands were prepared by chemoentzymatic synthesis. Novel selective glycopolymers were prepared that had a high affinity for Gal-1 or Gal-3. Selected glycopolymers were tested in new biological studies. In sum, seven publications were published, all with international co-authorship; one publication is in print.
Solution timeline
Realization period - beginning
Jun 1, 2017
Realization period - end
Apr 15, 2020
Project status
U - Finished project
Latest support payment
Feb 24, 2020
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP21-MSM-LT-U/01:1
Data delivery date
Jun 15, 2021
Finance
Total approved costs
2,791 thou. CZK
Public financial support
2,791 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK