Zapojení jaderného Vinkulínu do ubikvitinační dráhy vedoucí k proteazomu v průběhu ranné meiózy.

Název projektu anglicky
Involvement of nuclear Vinculin in ubiquitin relay of proteasomes during early meiotic events.
Anotace anglicky
Meiosis is a key process for sexual reproduction contributing to the genetic variability. During meiosis, homologous chromosomes must find their partners and they undergo dramatic motions, finally stabilized by synaptonemal complexes. The ubiquitin-proteasome system (UPS) is a key regulators of cellular proteostasis. It is implicated in various aspects of meiotic prophase. However, some points of its functioning in mammalian meiosis remain poorly characterized. Our preliminary data have shown that protein Vinculin (VCL) may be involved in UPS regulation. Our preliminary experiments showed an altered meiotic progression and synapsis defects in spermatocytes with VCL conditional knock-out. In addition, mass-spectrometry analysis of VCL-interacting proteins in prophase I nuclei revealed the prominent part of the proteasome regulation part as well as ubiquitin ligases and de-ubiquitinases. We propose therefore this project which aims to clarify the involvement of VCL in processes of protein-ubiquitin modification and their direction to the proteasome during meiotic Prophase I.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10601 - Cell biology
OECD FORD - vedlejší obor
10604 - Reproductive biology (medical aspects to be 3)
OECD FORD - další vedlejší obor
10608 - Biochemistry and molecular biology
CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
CE - Biochemie<br>EA - Morfologické obory a cytologie<br>EB - Genetika a molekulární biologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP22-GA0-GA-R
Datum dodání záznamu
22. 2. 2022

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