Chemická modulace aktivity Ku komplexu a využití v editaci genomů.

Název projektu anglicky
Chemical modulation of Ku activity and its use in genome editing.
Anotace anglicky
Genome integrity is governed by multiple mechanisms among which the pathways dealing with broken DNA ends are the most complex. Due to its strong DNA-end binding activity, Ku is one of the key molecules involved in metabolism of DNA breaks. It is essential for DNA repair via non-homologous end joining, but it is also required for integrity of telomeres. Owing to its important function in genome maintenance, exploration of Ku opens interesting possibilities in biotechnology and medicine. So far, Ku function has been modulated genetically as no chemical inhibitors are available. Here we propose to study mechanism of Ku-DNA interaction with emphasis on the C-terminal SAP domain of Ku70. In addition, we want to perform screening of chemical libraries to identify compounds that inhibit Ku association with DNA. The identified inhibitors will be biochemically characterized and tested for their biological activity in vivo. Finally, we will determine whether these compounds alter efficiency of gene targeting and spectrum of mutations induced by CRISPR/Cas9 in plant cells.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10608 - Biochemistry and molecular biology
OECD FORD - vedlejší obor
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OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
CE - Biochemie<br>EB - Genetika a molekulární biologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP22-GA0-GA-R
Datum dodání záznamu
22. 2. 2022

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