Identification of virulence factors of Treponema pallidum subspecies pallidum: from human transcriptomic data to in vitro characterization of strains

Název projektu anglicky
Identification of virulence factors of Treponema pallidum subspecies pallidum: from human transcriptomic data to in vitro characterization of strains
Anotace anglicky
The infections caused by Treponema pallidum subsp. pallidum (TPA) represent a serious health problem with increasing number of new syphilis cases worldwide. In this study, we are planning to perform first transcriptomic studies on TPA-containing samples isolated directly from the infected patients and compare them to the transcriptomes obtained during in vitro cultivation of the same TPA strains derived from clinical material. Gene expression differences will be used to identify genes potentially encoding virulence determinants. A directed mutagenesis will be employed to prepare deletion mutants that will be tested in vitro for TPA growth and interactions with human cells by monitoring of several eukaryotic signaling pathways. The identified TPA proteins involved in interaction with human eukaryotic cells will be considered as possible virulence factors representing potential targets to develop new therapeutics and/or vaccine against syphilis.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10606 - Microbiology
OECD FORD - vedlejší obor
30303 - Infectious Diseases
OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
EE - Mikrobiologie, virologie<br>FN - Epidemiologie, infekční nemoci a klinická imunologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP26-GA0-GA-R
Datum dodání záznamu
4. 5. 2026

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