HNO3-PCF: A new lossless light microscopy method for forensic diatom drowning analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F25%3AN0000001" target="_blank" >RIV/00007064:K01__/25:N0000001 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0379073825000878?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0379073825000878?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.forsciint.2025.112449" target="_blank" >10.1016/j.forsciint.2025.112449</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
HNO3-PCF: A new lossless light microscopy method for forensic diatom drowning analysis
Popis výsledku v původním jazyce
Five simple methods for diatom drowning analysis, including three newly developed ones, were compared for diatom yield and species composition consistency. Saponification of fats using sodium hydroxide solution significantly improved the yield of the extraction step, as well as the use of filtration instead of centrifugation. The combination of nitric acid digestion, vacuum filtration, and polycarbonate filter melting led to the development of the lossless light microscopy diatom forensic method (HNO3-PCF), with no detectable alteration in diatom community composition. When applied to 4-week submerged pig cadavers and six confirmed drowned human cases, the method exhibited unprecedented sensitivity. Among eight tissue types, lung diatom concentration was found to be the only reliable indicator for distinguishing between non-drowned and drowned cases. To minimize false positives from systematic contamination of the thoracic cavity, subtraction of heart diatom concentration from lung values was recommended. Provisional diatom concentration thresholds for drowning determination were set. The method’s low equipment requirements (simple vacuum filtration, light microscope, hot plate) and high efficiency make it a promising tool for widespread use in forensic practice.
Název v anglickém jazyce
HNO3-PCF: A new lossless light microscopy method for forensic diatom drowning analysis
Popis výsledku anglicky
Five simple methods for diatom drowning analysis, including three newly developed ones, were compared for diatom yield and species composition consistency. Saponification of fats using sodium hydroxide solution significantly improved the yield of the extraction step, as well as the use of filtration instead of centrifugation. The combination of nitric acid digestion, vacuum filtration, and polycarbonate filter melting led to the development of the lossless light microscopy diatom forensic method (HNO3-PCF), with no detectable alteration in diatom community composition. When applied to 4-week submerged pig cadavers and six confirmed drowned human cases, the method exhibited unprecedented sensitivity. Among eight tissue types, lung diatom concentration was found to be the only reliable indicator for distinguishing between non-drowned and drowned cases. To minimize false positives from systematic contamination of the thoracic cavity, subtraction of heart diatom concentration from lung values was recommended. Provisional diatom concentration thresholds for drowning determination were set. The method’s low equipment requirements (simple vacuum filtration, light microscope, hot plate) and high efficiency make it a promising tool for widespread use in forensic practice.
Klasifikace
Druh
J<sub>ost</sub> - Ostatní články v recenzovaných periodicích
CEP obor
—
OECD FORD obor
10620 - Other biological topics
Návaznosti výsledku
Projekt
<a href="/cs/project/VK01010069" target="_blank" >VK01010069: Využití analýzy druhového složení společenstev rozsivek (Bacillariophyceae) ve forenzní praxi.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Forensic Science International
ISSN
0379-0738
e-ISSN
1872-6283
Svazek periodika
370
Číslo periodika v rámci svazku
May 2025
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
112449
Kód UT WoS článku
—
EID výsledku v databázi Scopus
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