Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F14%3A00059055" target="_blank" >RIV/00023001:_____/14:00059055 - isvavai.cz</a>
Výsledek na webu
<a href="http://annalsoftransplantation.com/abstract/index/idArt/889870" target="_blank" >http://annalsoftransplantation.com/abstract/index/idArt/889870</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.12659/AOT.889870" target="_blank" >10.12659/AOT.889870</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats
Popis výsledku v původním jazyce
Background: Arterial allografts are used as vascular conduits in the treatment of prosthetic graft infection. Immunosuppression decreases their rupture risk rate. However, immunosuppression can be unprofitable in florid infection. Previously, we confirmed inhibition of cell-mediated destruction of rat aortic grafts by delayed use of tacrolimus. In this work, we studied the influence of this protocol on the antibody-mediated rejection. Material/Methods: Flow cytometry was used for the retrospective analysis of day 0, 14, and 30 sera obtained from Lewis rat recipients of isogeneic fresh infrarenal aortic grafts (group A) or Brown-Norway rat aortic grafts (group B, C, D) for the presence of donor-specific anti-MHC class I and II antibodies. Tacrolimus indaily dose of 0.2 mg/kg was administered from day 1 to day 30 (group C) or from day 7 to day 30 (group D). Results: Inhibition of fluorescence-labeled anti-BN-MHC class I and MHC class II antibodies binding to BN-splenocytes was observed
Název v anglickém jazyce
Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats
Popis výsledku anglicky
Background: Arterial allografts are used as vascular conduits in the treatment of prosthetic graft infection. Immunosuppression decreases their rupture risk rate. However, immunosuppression can be unprofitable in florid infection. Previously, we confirmed inhibition of cell-mediated destruction of rat aortic grafts by delayed use of tacrolimus. In this work, we studied the influence of this protocol on the antibody-mediated rejection. Material/Methods: Flow cytometry was used for the retrospective analysis of day 0, 14, and 30 sera obtained from Lewis rat recipients of isogeneic fresh infrarenal aortic grafts (group A) or Brown-Norway rat aortic grafts (group B, C, D) for the presence of donor-specific anti-MHC class I and II antibodies. Tacrolimus indaily dose of 0.2 mg/kg was administered from day 1 to day 30 (group C) or from day 7 to day 30 (group D). Results: Inhibition of fluorescence-labeled anti-BN-MHC class I and MHC class II antibodies binding to BN-splenocytes was observed
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FJ - Chirurgie včetně transplantologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Annals of transplantation
ISSN
1425-9524
e-ISSN
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Svazek periodika
19
Číslo periodika v rámci svazku
may
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
8
Strana od-do
225-232
Kód UT WoS článku
000342976000001
EID výsledku v databázi Scopus
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