Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378504" target="_blank" >RIV/00216208:11110/18:10378504 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/18:10378504 RIV/00064165:_____/18:10378504

Výsledek na webu

<a href="https://doi.org/10.1371/journal.pone.0201984" target="_blank" >https://doi.org/10.1371/journal.pone.0201984</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0201984" target="_blank" >10.1371/journal.pone.0201984</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats

Popis výsledku v původním jazyce

Objectives and design: The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. Materials and methods: Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the analysis of day 30 sera for the presence of donor specific antiMHC class I and II antibodies. Results: The aortal allografts in both immunosuppressed groups showed regular morphology of aortal wall with no depositions of immunoglobulin G on day 30. The adventitial infiltration of non-immunosuppressed aortal allografts by MHC class II positive cells of recipient origin was significantly higher (ALO 20.7 +/- 6.7 cells, P&lt;0.001) compared to both immunosuppressed groups (TAC1 5.9 +/- 5.5 cells, TAC7 6.1 +/- 5.1 cells). Day 30 sera from the allogeneic non-immunosuppressed animals decreased significantly the binding of fluorescence labelled MHC class I (46.9 +/- 19.4%) and class II (65.8 +/- 11.9%) antibody to donors spleen cells compared with day 30 sera from both immunosuppressed groups (TAC1, anti-MHC class 1102.4 +/- 4.2%, p &lt; 0.001, anti-MHC class II 102.6 +/- 6.0%), (TAC7, anti-MHC class I 79.9 +/- 3.3%, p &lt; 0.001, anti-MHC class II 80.9 +/- 2.7%). Conclusion: Both immunosuppressed protocols with tacrolimus (administration from day 1 or from day 7 following transplantation) were able to suppress acute cell- and antibody-mediated rejection of cryopreserved abdominal aortic allografts processed in accordance with our new standardized clinical protocol.

Název v anglickém jazyce

Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats

Popis výsledku anglicky

Objectives and design: The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. Materials and methods: Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the analysis of day 30 sera for the presence of donor specific antiMHC class I and II antibodies. Results: The aortal allografts in both immunosuppressed groups showed regular morphology of aortal wall with no depositions of immunoglobulin G on day 30. The adventitial infiltration of non-immunosuppressed aortal allografts by MHC class II positive cells of recipient origin was significantly higher (ALO 20.7 +/- 6.7 cells, P&lt;0.001) compared to both immunosuppressed groups (TAC1 5.9 +/- 5.5 cells, TAC7 6.1 +/- 5.1 cells). Day 30 sera from the allogeneic non-immunosuppressed animals decreased significantly the binding of fluorescence labelled MHC class I (46.9 +/- 19.4%) and class II (65.8 +/- 11.9%) antibody to donors spleen cells compared with day 30 sera from both immunosuppressed groups (TAC1, anti-MHC class 1102.4 +/- 4.2%, p &lt; 0.001, anti-MHC class II 102.6 +/- 6.0%), (TAC7, anti-MHC class I 79.9 +/- 3.3%, p &lt; 0.001, anti-MHC class II 80.9 +/- 2.7%). Conclusion: Both immunosuppressed protocols with tacrolimus (administration from day 1 or from day 7 following transplantation) were able to suppress acute cell- and antibody-mediated rejection of cryopreserved abdominal aortic allografts processed in accordance with our new standardized clinical protocol.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

—

Kód UT WoS článku

000441232600075

EID výsledku v databázi Scopus

2-s2.0-85052315026