Manganese-Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00077860" target="_blank" >RIV/00023001:_____/19:00077860 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/19:00517436 RIV/68378041:_____/19:00517436 RIV/60461373:22340/19:43917736 RIV/00216208:11110/19:10397522

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/open.201800261" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/open.201800261</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/open.201800261" target="_blank" >10.1002/open.201800261</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Manganese-Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

Popis výsledku v původním jazyce

Manganese-zinc ferrite nanoparticles were synthesized by using a hydrothermal treatment, coated with silica, and then tested as efficient cellular labels for cell tracking, using magnetic resonance imaging (MRI) in vivo. A toxicity study was performed on rat mesenchymal stem cells and C6 glioblastoma cells. Adverse effects on viability and cell proliferation were observed at the highest concentration (0.55 mM) only; cell viability was not compromised at lower concentrations. Nanoparticle internalization was confirmed by transmission electron microscopy. The particles were found in membranous vesicles inside the cytoplasm. Although the metal content (0.42 pg Fe/cell) was lower compared to commercially available iron oxide nanoparticles, labeled cells reached a comparable relaxation rate R-2, owing to higher nanoparticle relaxivity. Cells from transgenic luciferase-positive rats were used for in vivo experiments. Labeled cells were transplanted into the muscles of non bioluminescent rats and visualized by MRI. The cells produced a distinct hypointense signal in T-2 - or T-2*-weighted MR images in vivo. Cell viability in vivo was verified by bioluminescence.

Název v anglickém jazyce

Manganese-Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

Popis výsledku anglicky

Manganese-zinc ferrite nanoparticles were synthesized by using a hydrothermal treatment, coated with silica, and then tested as efficient cellular labels for cell tracking, using magnetic resonance imaging (MRI) in vivo. A toxicity study was performed on rat mesenchymal stem cells and C6 glioblastoma cells. Adverse effects on viability and cell proliferation were observed at the highest concentration (0.55 mM) only; cell viability was not compromised at lower concentrations. Nanoparticle internalization was confirmed by transmission electron microscopy. The particles were found in membranous vesicles inside the cytoplasm. Although the metal content (0.42 pg Fe/cell) was lower compared to commercially available iron oxide nanoparticles, labeled cells reached a comparable relaxation rate R-2, owing to higher nanoparticle relaxivity. Cells from transgenic luciferase-positive rats were used for in vivo experiments. Labeled cells were transplanted into the muscles of non bioluminescent rats and visualized by MRI. The cells produced a distinct hypointense signal in T-2 - or T-2*-weighted MR images in vivo. Cell viability in vivo was verified by bioluminescence.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemistryOpen

ISSN

2191-1363

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

155-165

Kód UT WoS článku

000463944800003

EID výsledku v databázi Scopus

2-s2.0-85060584830