Ultralong TE In Vivo H-1 MR spectroscopy of omega-3 fatty acids in subcutaneous adipose tissue at 7 T

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078051" target="_blank" >RIV/00023001:_____/19:00078051 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023752:_____/19:43920313

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmri.26605" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmri.26605</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jmri.26605" target="_blank" >10.1002/jmri.26605</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultralong TE In Vivo H-1 MR spectroscopy of omega-3 fatty acids in subcutaneous adipose tissue at 7 T

Popis výsledku v původním jazyce

Background Omega-3 (n-3) fatty acids (FA) play and important role in neural development and other metabolic diseases such as obesity and diabetes. The knowledge about the in vivo content and distribution of n-3 FA in human body tissues is not well established and the standard quantification of FA is invasive and costly. Purpose To detect omega-3 (n-3 CH3) and non-omega-3 (CH3) methyl group resonance lines with echo times up to 1200 msec, in oils, for the assessment of n-3 FA content, and the n-3 FA fraction in adipose tissue in vivo. Study Type Prospective technical development. Population Three oils with different n-3 FA content and 24 healthy subjects. Field Strength/Sequence Single-voxel MR spectroscopy (SVS) with a point-resolved spectroscopy (PRESS) sequence with an echo time (TE) of 1000 msec at 7 T. Assessment Knowledge about the J-coupling evolution of both CH3 resonances was used for the optimal detection of the n-3 CH3 resonance line at a TE of 1000 msec. The accuracy of the method in oils and in vivo was validated from a biopsy sample with gas chromatography analysis. Statistical Tests SVS data were compared to gas chromatography with the Pearson correlation coefficient. Results T-2 relaxation times in oils were assessed as follows: CH2, 65 +/- 22 msec; CH3, 325 +/- 7 msec; and n-3 CH3, 628 +/- 34 msec. The n-3 FA fractions from oil phantom experiments (n = 3) were in agreement with chromatography analysis and the comparison of in vivo obtained data with the results of chromatography analysis (n = 5) yielded a significant correlation (P = 0.029). Data Conclusion PRESS with ultralong-TE can detect and quantify the n-3 CH3 signal in vivo at 7 T. Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:71-82.

Název v anglickém jazyce

Ultralong TE In Vivo H-1 MR spectroscopy of omega-3 fatty acids in subcutaneous adipose tissue at 7 T

Popis výsledku anglicky

Background Omega-3 (n-3) fatty acids (FA) play and important role in neural development and other metabolic diseases such as obesity and diabetes. The knowledge about the in vivo content and distribution of n-3 FA in human body tissues is not well established and the standard quantification of FA is invasive and costly. Purpose To detect omega-3 (n-3 CH3) and non-omega-3 (CH3) methyl group resonance lines with echo times up to 1200 msec, in oils, for the assessment of n-3 FA content, and the n-3 FA fraction in adipose tissue in vivo. Study Type Prospective technical development. Population Three oils with different n-3 FA content and 24 healthy subjects. Field Strength/Sequence Single-voxel MR spectroscopy (SVS) with a point-resolved spectroscopy (PRESS) sequence with an echo time (TE) of 1000 msec at 7 T. Assessment Knowledge about the J-coupling evolution of both CH3 resonances was used for the optimal detection of the n-3 CH3 resonance line at a TE of 1000 msec. The accuracy of the method in oils and in vivo was validated from a biopsy sample with gas chromatography analysis. Statistical Tests SVS data were compared to gas chromatography with the Pearson correlation coefficient. Results T-2 relaxation times in oils were assessed as follows: CH2, 65 +/- 22 msec; CH3, 325 +/- 7 msec; and n-3 CH3, 628 +/- 34 msec. The n-3 FA fractions from oil phantom experiments (n = 3) were in agreement with chromatography analysis and the comparison of in vivo obtained data with the results of chromatography analysis (n = 5) yielded a significant correlation (P = 0.029). Data Conclusion PRESS with ultralong-TE can detect and quantify the n-3 CH3 signal in vivo at 7 T. Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:71-82.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of magnetic resonance imaging

ISSN

1053-1807

e-ISSN

—

Svazek periodika

50

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

71-82

Kód UT WoS článku

000471831600006

EID výsledku v databázi Scopus

2-s2.0-85058969321