The in vivo J-difference editing MEGA-PRESS technique for the detection of n-3 fatty acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10287036" target="_blank" >RIV/00216208:11310/14:10287036 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/14:00059101

Výsledek na webu

<a href="http://dx.doi.org/10.1002/nbm.3189" target="_blank" >http://dx.doi.org/10.1002/nbm.3189</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/nbm.3189" target="_blank" >10.1002/nbm.3189</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The in vivo J-difference editing MEGA-PRESS technique for the detection of n-3 fatty acids

Popis výsledku v původním jazyce

In this study, we present a method for the detection of n-3 fatty acid (n-3 FA) signals using MRS in adipose tissue in vivo. This method (called oMEGA-PRESS) is based on the selective detection of the CH3 signal of n-3 FA using the MEGA-PRESS (MEshcher-GArwood Point-RESolved Spectroscopy) J-difference editing technique. We optimized the envelope shape and frequency of spectral editing pulses to minimize the spurious co-editing and incomplete subtraction of the CH3 signal of other FAs, which normally obscure the n-3 FA CH3 signal in MR spectra acquired using standard PRESS techniques. The post-processing of the individual data scans with the phase and frequency correction before data subtraction and averaging was implemented to further improve the quality of in vivo spectra. The technique was optimized in vitro on lipid phantoms using various concentrations of n-3 FA and examined in vivo at 3 T on 15 healthy volunteers. The proportion of n-3 FA estimated by the oMEGA-PRESS method in pha

Název v anglickém jazyce

The in vivo J-difference editing MEGA-PRESS technique for the detection of n-3 fatty acids

Popis výsledku anglicky

In this study, we present a method for the detection of n-3 fatty acid (n-3 FA) signals using MRS in adipose tissue in vivo. This method (called oMEGA-PRESS) is based on the selective detection of the CH3 signal of n-3 FA using the MEGA-PRESS (MEshcher-GArwood Point-RESolved Spectroscopy) J-difference editing technique. We optimized the envelope shape and frequency of spectral editing pulses to minimize the spurious co-editing and incomplete subtraction of the CH3 signal of other FAs, which normally obscure the n-3 FA CH3 signal in MR spectra acquired using standard PRESS techniques. The post-processing of the individual data scans with the phase and frequency correction before data subtraction and averaging was implemented to further improve the quality of in vivo spectra. The technique was optimized in vitro on lipid phantoms using various concentrations of n-3 FA and examined in vivo at 3 T on 15 healthy volunteers. The proportion of n-3 FA estimated by the oMEGA-PRESS method in pha

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GPP208%2F11%2FP059" target="_blank" >GPP208/11/P059: Nové techniky in-vivo MR spektroskopie pro studium metabolismu triacylglycerolů tukové tkáně</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NMR in Biomedicine

ISSN

0952-3480

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

1293-1299

Kód UT WoS článku

000343927900004

EID výsledku v databázi Scopus

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