Ultralong TE In Vivo H-1 MR Spectroscopy of Omega-3 Fatty Acids in Subcutaneous Adipose Tissue at 7 T

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F19%3A43920313" target="_blank" >RIV/00023752:_____/19:43920313 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/19:00078051

Výsledek na webu

<a href="https://www.researchgate.net/publication/329869555_Ultralong_TE_In_Vivo_1_H_MR_Spectroscopy_of_Omega-3_Fatty_Acids_in_Subcutaneous_Adipose_Tissue_at_7_T_Omega-3_Fatty_Acids_Detection_at_7_T" target="_blank" >https://www.researchgate.net/publication/329869555_Ultralong_TE_In_Vivo_1_H_MR_Spectroscopy_of_Omega-3_Fatty_Acids_in_Subcutaneous_Adipose_Tissue_at_7_T_Omega-3_Fatty_Acids_Detection_at_7_T</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jmri.26605" target="_blank" >10.1002/jmri.26605</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultralong TE In Vivo H-1 MR Spectroscopy of Omega-3 Fatty Acids in Subcutaneous Adipose Tissue at 7 T

Popis výsledku v původním jazyce

Background Omega‐3 (n‐3) fatty acids (FA) play and important role in neural development and other metabolic diseases such as obesity and diabetes. The knowledge about the in vivo content and distribution of n‐3 FA in human body tissues is not well established and the standard quantification of FA is invasive and costly. Purpose To detect omega‐3 (n‐3 CH3) and non‐omega‐3 (CH3) methyl group resonance lines with echo times up to 1200 msec, in oils, for the assessment of n‐3 FA content, and the n‐3 FA fraction in adipose tissue in vivo. Study Type Prospective technical development. Population Three oils with different n‐3 FA content and 24 healthy subjects. Field Strength/Sequence Single‐voxel MR spectroscopy (SVS) with a point‐resolved spectroscopy (PRESS) sequence with an echo time (TE) of 1000 msec at 7 T. Assessment Knowledge about the J‐coupling evolution of both CH3 resonances was used for the optimal detection of the n‐3 CH3 resonance line at a TE of 1000 msec. The accuracy of the method in oils and in vivo was validated from a biopsy sample with gas chromatography analysis. Statistical Tests SVS data were compared to gas chromatography with the Pearson correlation coefficient. Results T2 relaxation times in oils were assessed as follows: CH2, 65 ± 22 msec; CH3, 325 ± 7 msec; and n‐3 CH3, 628 ± 34 msec. The n‐3 FA fractions from oil phantom experiments (n = 3) were in agreement with chromatography analysis and the comparison of in vivo obtained data with the results of chromatography analysis (n = 5) yielded a significant correlation (P = 0.029). Data Conclusion PRESS with ultralong‐TE can detect and quantify the n‐3 CH3 signal in vivo at 7 T. Level of Evidence 1 Technical Efficacy Stage 1

Název v anglickém jazyce

Ultralong TE In Vivo H-1 MR Spectroscopy of Omega-3 Fatty Acids in Subcutaneous Adipose Tissue at 7 T

Popis výsledku anglicky

Background Omega‐3 (n‐3) fatty acids (FA) play and important role in neural development and other metabolic diseases such as obesity and diabetes. The knowledge about the in vivo content and distribution of n‐3 FA in human body tissues is not well established and the standard quantification of FA is invasive and costly. Purpose To detect omega‐3 (n‐3 CH3) and non‐omega‐3 (CH3) methyl group resonance lines with echo times up to 1200 msec, in oils, for the assessment of n‐3 FA content, and the n‐3 FA fraction in adipose tissue in vivo. Study Type Prospective technical development. Population Three oils with different n‐3 FA content and 24 healthy subjects. Field Strength/Sequence Single‐voxel MR spectroscopy (SVS) with a point‐resolved spectroscopy (PRESS) sequence with an echo time (TE) of 1000 msec at 7 T. Assessment Knowledge about the J‐coupling evolution of both CH3 resonances was used for the optimal detection of the n‐3 CH3 resonance line at a TE of 1000 msec. The accuracy of the method in oils and in vivo was validated from a biopsy sample with gas chromatography analysis. Statistical Tests SVS data were compared to gas chromatography with the Pearson correlation coefficient. Results T2 relaxation times in oils were assessed as follows: CH2, 65 ± 22 msec; CH3, 325 ± 7 msec; and n‐3 CH3, 628 ± 34 msec. The n‐3 FA fractions from oil phantom experiments (n = 3) were in agreement with chromatography analysis and the comparison of in vivo obtained data with the results of chromatography analysis (n = 5) yielded a significant correlation (P = 0.029). Data Conclusion PRESS with ultralong‐TE can detect and quantify the n‐3 CH3 signal in vivo at 7 T. Level of Evidence 1 Technical Efficacy Stage 1

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Magnetic Resonance Imaging

ISSN

1053-1807

e-ISSN

—

Svazek periodika

50

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

71-82

Kód UT WoS článku

000471831600006

EID výsledku v databázi Scopus

2-s2.0-85058969321