The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078395" target="_blank" >RIV/00023001:_____/19:00078395 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10408776

Výsledek na webu

<a href="https://www.tandfonline.com/doi/pdf/10.1080/19336918.2019.1644856?needAccess=true" target="_blank" >https://www.tandfonline.com/doi/pdf/10.1080/19336918.2019.1644856?needAccess=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19336918.2019.1644856" target="_blank" >10.1080/19336918.2019.1644856</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium

Popis výsledku v původním jazyce

Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro-inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1 beta,TNF-alpha,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1 beta and TNF-alpha in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1 beta, TNF-alpha, MCP-1, IL-10, and RANTES, with IL-1 beta and TNF-alpha having the strongest impact.

Název v anglickém jazyce

The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium

Popis výsledku anglicky

Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro-inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1 beta,TNF-alpha,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1 beta and TNF-alpha in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1 beta, TNF-alpha, MCP-1, IL-10, and RANTES, with IL-1 beta and TNF-alpha having the strongest impact.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell adhesion and migration

ISSN

1933-6918

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

293-302

Kód UT WoS článku

000485070900001

EID výsledku v databázi Scopus

2-s2.0-85075981010