Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F24%3A00085207" target="_blank" >RIV/00023001:_____/24:00085207 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/chapter/10.1007/978-3-031-62520-6_45" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-62520-6_45</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_45" target="_blank" >10.1007/978-3-031-62520-6_45</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku v původním jazyce

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton’s jelly stromal cells. Decellularization was held in a custom-built automated system allowing cyclic change of decellularization agents and cleaning water, maintaining a reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting methods. Porcine collagen with incorporated cells was used. Then to coat the inner lumen of tubular tissue microextrusion method was created. It uses special microcannulas where collagen with cells was extruded, leaving inner lumen homogeneously coated. Prepared planar patches and tubular grafts were then implanted into pigs for one month observation. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.

Název v anglickém jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku anglicky

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton’s jelly stromal cells. Decellularization was held in a custom-built automated system allowing cyclic change of decellularization agents and cleaning water, maintaining a reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting methods. Porcine collagen with incorporated cells was used. Then to coat the inner lumen of tubular tissue microextrusion method was created. It uses special microcannulas where collagen with cells was extruded, leaving inner lumen homogeneously coated. Prepared planar patches and tubular grafts were then implanted into pigs for one month observation. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

30212 - Surgery

Projekt

<a href="/cs/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificiální kardiovaskulární záplaty a cévní náhrady na bázi porcinního kolagenu zesílené nano/mikrovlákny remodelované pomocí kmenových buněk v bioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Advances in Digital Health and Medical Bioengineering : IFMBE Proceedings

ISBN

978-3-031-62519-0

Počet stran výsledku

8

Strana od-do

406-413

Počet stran knihy

696

Název nakladatele

Springer

Místo vydání

Cham

Kód UT WoS kapitoly

001326809000045