Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F24%3A00604266" target="_blank" >RIV/67985891:_____/24:00604266 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-3-031-62520-6_45" target="_blank" >https://doi.org/10.1007/978-3-031-62520-6_45</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_45" target="_blank" >10.1007/978-3-031-62520-6_45</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku v původním jazyce

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton's jelly stromal cells. Decellularization was held in a custom-built automated system allowing cyclic change of decellularization agents and cleaning water, maintaining a reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting methods. Porcine collagen with incorporated cells was used. Then to coat the inner lumen of tubular tissue microextrusion method was created. It uses special microcannulas where collagen with cells was extruded, leaving inner lumen homogeneously coated. Prepared planar patches and tubular grafts were then implanted into pigs for one month observation.

Název v anglickém jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku anglicky

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton's jelly stromal cells. Decellularization was held in a custom-built automated system allowing cyclic change of decellularization agents and cleaning water, maintaining a reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting methods. Porcine collagen with incorporated cells was used. Then to coat the inner lumen of tubular tissue microextrusion method was created. It uses special microcannulas where collagen with cells was extruded, leaving inner lumen homogeneously coated. Prepared planar patches and tubular grafts were then implanted into pigs for one month observation.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificiální kardiovaskulární záplaty a cévní náhrady na bázi porcinního kolagenu zesílené nano/mikrovlákny remodelované pomocí kmenových buněk v bioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Advances in digital health and medical bioengineering, VOL 2, EHB-2023

ISBN

978-3-031-62520-6

ISSN

1680-0737

e-ISSN

—

Počet stran výsledku

8

Strana od-do

406-413

Název nakladatele

Springer

Místo vydání

Cham

Místo konání akce

Bucharest

Datum konání akce

9. 11. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001326809000045