Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F24%3A00371292" target="_blank" >RIV/68407700:21460/24:00371292 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_45" target="_blank" >http://dx.doi.org/10.1007/978-3-031-62520-6_45</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_45" target="_blank" >10.1007/978-3-031-62520-6_45</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku v původním jazyce

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton’s jelly stromal cells. Decellularization was held in custom build automated system allowing cyclic change of decellularization agents and cleaning water maintaining reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting method. Porcine collagen with incorporated cells was used. Then to coat inner lumen of tubular tissue microextrusion method was created. It uses special microcanulas where collagen with cells was extruded, leaving inner lumen homogenously coated. Prepared planar patches and tubular graft were then implanted into pigs for one month observation.

Název v anglickém jazyce

Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting

Popis výsledku anglicky

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton’s jelly stromal cells. Decellularization was held in custom build automated system allowing cyclic change of decellularization agents and cleaning water maintaining reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting method. Porcine collagen with incorporated cells was used. Then to coat inner lumen of tubular tissue microextrusion method was created. It uses special microcanulas where collagen with cells was extruded, leaving inner lumen homogenously coated. Prepared planar patches and tubular graft were then implanted into pigs for one month observation.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

<a href="/cs/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificiální kardiovaskulární záplaty a cévní náhrady na bázi porcinního kolagenu zesílené nano/mikrovlákny remodelované pomocí kmenových buněk v bioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Advances in Digital Health and Medical Bioengineering, Proceedings of the 11th International Conference on E-Health and Bioengineering, EHB-2023, November 9–10, 2023, Bucharest, Romania – Volume 2: Health Technology Assessment, Biomedical Signal Processing, Medicine and Informatics

ISBN

978-3-031-62519-0

ISSN

1680-0737

e-ISSN

1433-9277

Počet stran výsledku

8

Strana od-do

406-413

Název nakladatele

Springer Nature Switzerland AG

Místo vydání

Basel

Místo konání akce

Bucuresti

Datum konání akce

9. 11. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001326809000045