Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F11%3A00009312" target="_blank" >RIV/00023736:_____/11:00009312 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/jcb.23263" target="_blank" >http://dx.doi.org/10.1002/jcb.23263</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.23263" target="_blank" >10.1002/jcb.23263</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes
Popis výsledku v původním jazyce
The effects of the pan-caspase inhibitor Q-VD-OPh on caspase activity, DNA fragmentation, PARP cleavage, 7A6 exposition, and cellular adhesivity to fibronectin were analyzed in three different apoptotic systems involving two cell lines and two apoptosisinducers. Q-VD-OPh fully inhibited caspase-3 and -7 activity at 0.05 ?M concentration, but significantly higher Q-VD-OPh dose (10 ?M) was required to prevent the cleavage of PARP-1. DNA fragmentation and cell membrane permeabilization were both preventedat 2 ?M Q-VD-OPh while 10 ?M inhibitor was needed to inhibit the loss of cellular adhesivity to fibronectin. The exposition of the mitochondrial antigen 7A6 occurred independently of Q-VD-OPh addition and may serve to the detection of cumulative incidence of the cells which have initiated the apoptosis. Q-VD-OPh efficiency in the whole-cell environment is of two orders of magnitude higher than that of z-VAD-fmk and this is not due to a low permeability of the latter through the membrane
Název v anglickém jazyce
Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes
Popis výsledku anglicky
The effects of the pan-caspase inhibitor Q-VD-OPh on caspase activity, DNA fragmentation, PARP cleavage, 7A6 exposition, and cellular adhesivity to fibronectin were analyzed in three different apoptotic systems involving two cell lines and two apoptosisinducers. Q-VD-OPh fully inhibited caspase-3 and -7 activity at 0.05 ?M concentration, but significantly higher Q-VD-OPh dose (10 ?M) was required to prevent the cleavage of PARP-1. DNA fragmentation and cell membrane permeabilization were both preventedat 2 ?M Q-VD-OPh while 10 ?M inhibitor was needed to inhibit the loss of cellular adhesivity to fibronectin. The exposition of the mitochondrial antigen 7A6 occurred independently of Q-VD-OPh addition and may serve to the detection of cumulative incidence of the cells which have initiated the apoptosis. Q-VD-OPh efficiency in the whole-cell environment is of two orders of magnitude higher than that of z-VAD-fmk and this is not due to a low permeability of the latter through the membrane
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA301%2F09%2F1026" target="_blank" >GA301/09/1026: Proteomová analýza účinků suberoylanilidu hydroxamové kyseliny (SAHA) na lidské leukemické buňky</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Cellular Biochemistry
ISSN
0730-2312
e-ISSN
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Svazek periodika
112
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
3334-3342
Kód UT WoS článku
000296423000028
EID výsledku v databázi Scopus
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