Unraveling the complexity of tyrosine kinase inhibitor-resistant populations by ultra-deep sequencing of the BCR-ABL kinase domain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F13%3A00010715" target="_blank" >RIV/00023736:_____/13:00010715 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1182/blood-2013-03-487728" target="_blank" >http://dx.doi.org/10.1182/blood-2013-03-487728</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1182/blood-2013-03-487728" target="_blank" >10.1182/blood-2013-03-487728</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unraveling the complexity of tyrosine kinase inhibitor-resistant populations by ultra-deep sequencing of the BCR-ABL kinase domain

Popis výsledku v původním jazyce

We found that conventional Sanger sequencing had misclassified or underestimated BCR-ABL mutation status in 55% of the samples, where mutations with 1-15% abundance were detected. A complex clonal texture was uncovered by clonal analysis of samples harbouring multiple mutations and up to thirteen different mutated populations were identified. The landscape of these mutated populations was found to be highly dynamic. The high degree of complexity uncovered by UDS indicates that conventional Sanger sequencing might be an inadequate tool to assess BCR-ABL KD mutation status, that currently represents an important component of the therapeutic decision algorithms. Further evaluation of the clinical usefulness of UDS-based approaches is warranted.

Název v anglickém jazyce

Unraveling the complexity of tyrosine kinase inhibitor-resistant populations by ultra-deep sequencing of the BCR-ABL kinase domain

Popis výsledku anglicky

We found that conventional Sanger sequencing had misclassified or underestimated BCR-ABL mutation status in 55% of the samples, where mutations with 1-15% abundance were detected. A complex clonal texture was uncovered by clonal analysis of samples harbouring multiple mutations and up to thirteen different mutated populations were identified. The landscape of these mutated populations was found to be highly dynamic. The high degree of complexity uncovered by UDS indicates that conventional Sanger sequencing might be an inadequate tool to assess BCR-ABL KD mutation status, that currently represents an important component of the therapeutic decision algorithms. Further evaluation of the clinical usefulness of UDS-based approaches is warranted.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

<a href="/cs/project/NT11555" target="_blank" >NT11555: DNA fingerprinting u chronické myeloidní leukémie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Blood

ISSN

0006-4971

e-ISSN

—

Svazek periodika

122

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1634-1648

Kód UT WoS článku

000324056500018

EID výsledku v databázi Scopus

—