Indukce nemukoidních variant Clavibacter michiganensis subsp. sepedonicus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F02%3A925" target="_blank" >RIV/00027006:_____/02:925 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Induction of nonmucoid variants of Clavibacter michiganensis subsp. sepedonicus and comparison of their immunochemical and biochemical characteristics

Popis výsledku v původním jazyce

A total of 38 mucoid strains of Clavibacter michiganensis subsp. sepedonicus (Cms) were grown on culture media under different conditions e.g., of sugar concentration and temperatures with the aim to specify factors, which induce or stimulate a change ofcolony morphology from the mucoid wild type to nonmucoid form. It was found that stress conditions during cultivation, i. e. temperatures above 28°C (29-31°C), and culture media containing lower quantity of sugars, i.e. less than recommended level, stimulated the changes in colony morphology. The degree of mucoid growth of Cms strains decreased under the influence of these stress factors. Nevertheless, the influence of tested factors was not sufficient to change mucoid strains irreversibly to nonmucoidones. Partially nonmucoid variants were termed "intermediate" in colony morphology. Using DAS-ELISA and Biolog GP MicroPlate SystemTM, mucoid and intermediate variants of Cms were tested to see if these techniques might serve to identify

Název v anglickém jazyce

Induction of nonmucoid variants of Clavibacter michiganensis subsp. sepedonicus and comparison of their immunochemical and biochemical characteristics

Popis výsledku anglicky

A total of 38 mucoid strains of Clavibacter michiganensis subsp. sepedonicus (Cms) were grown on culture media under different conditions e.g., of sugar concentration and temperatures with the aim to specify factors, which induce or stimulate a change ofcolony morphology from the mucoid wild type to nonmucoid form. It was found that stress conditions during cultivation, i. e. temperatures above 28°C (29-31°C), and culture media containing lower quantity of sugars, i.e. less than recommended level, stimulated the changes in colony morphology. The degree of mucoid growth of Cms strains decreased under the influence of these stress factors. Nevertheless, the influence of tested factors was not sufficient to change mucoid strains irreversibly to nonmucoidones. Partially nonmucoid variants were termed "intermediate" in colony morphology. Using DAS-ELISA and Biolog GP MicroPlate SystemTM, mucoid and intermediate variants of Cms were tested to see if these techniques might serve to identify

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GA - Zemědělská ekonomie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: Analýza genetické a patogenní variability v populaci Clavibacter michiganensis subsp. sepedonicus molekulárními technikami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Diseases and Protection

ISSN

1861-3829

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

109

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

630-638

Kód UT WoS článku

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EID výsledku v databázi Scopus

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