Možnosti detekce Pyrenophora teres v pletivech listů ječmene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A2423" target="_blank" >RIV/00027006:_____/04:2423 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Possibilities of Pyrenophora teres detection in barley leaf tissue

Popis výsledku v původním jazyce

Specific polymerase chain reaction (PCR) primers were developed from AFLP bands of DNA of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed to specifically amplify DNA from P. teres f.sp. teres and allow its differentiation from P. teres f.sp. maculata, which is morphologically very similar to P. teres f.sp. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungi mycelium. The PCR assay was validated with 60 samples from 10 barley hosts originating from several regions across the Czech Republic. No cross reaction was observed with DNA of several other species like P. tritici repentis, P. graminea and Helminthosporium sativum. This method is prepared to be used to detect the pathogen from environmental samples for survey and management purposes.

Název v anglickém jazyce

Possibilities of Pyrenophora teres detection in barley leaf tissue

Popis výsledku anglicky

Specific polymerase chain reaction (PCR) primers were developed from AFLP bands of DNA of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed to specifically amplify DNA from P. teres f.sp. teres and allow its differentiation from P. teres f.sp. maculata, which is morphologically very similar to P. teres f.sp. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungi mycelium. The PCR assay was validated with 60 samples from 10 barley hosts originating from several regions across the Czech Republic. No cross reaction was observed with DNA of several other species like P. tritici repentis, P. graminea and Helminthosporium sativum. This method is prepared to be used to detect the pathogen from environmental samples for survey and management purposes.

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QC1361" target="_blank" >QC1361: Vývoj metody pro kvantifikaci houbových patogenů na bázi RealTime PCR</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of 9-th International Barley Genetics Symposium

ISBN

80-902545-9-4

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

705-708

Název nakladatele

Agricultural Research Institute Kromeriz

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

20. 6. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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