Specifita alpha amylázového inhibitoru s Phaseolus vulgaris a jeho insekticidní a akaricidní aktivita.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A212" target="_blank" >RIV/00027006:_____/05:212 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inhibitory specificity and insecticidal selectivity of alfa-amylase inhibitor from Phaseolus vulgaris

Popis výsledku v původním jazyce

The primary structure and proteolytic processing of the alfa-amylase isoinhibitor alfaAI-1 from common bean (Phaseolus vulgaris cv. Magna) was determined by protein chemistry techniques. The inhibitory specificity of alfaAI-1 was screened with a panel orthe diges- tive alfa-amylases from 30 species of insects, mites, gastropod, annelid worm, nematode and fungal phytopathogens with afrocus on agricultural pests and important model species. This in vitro analysis showed a selective inhibition of alfa-amylases from three orders or insect (Coleoptera, Hymenoptera and Diptera) and an inhibition of alfa-amylases of the annelid worm. The inhibitory potential of alfaAI-1 against several alfa-amylases was found to be modulated by pH. To understand how alfaAI-1discriminates among closely related alfa-amylases, the sequences of the alfa-amylases sensitive, respectively, insensitive to alfaAI-1 were compared, and the critical determinants were localized on the spatial alfa-amylase model. Based o

Název v anglickém jazyce

Inhibitory specificity and insecticidal selectivity of alfa-amylase inhibitor from Phaseolus vulgaris

Popis výsledku anglicky

The primary structure and proteolytic processing of the alfa-amylase isoinhibitor alfaAI-1 from common bean (Phaseolus vulgaris cv. Magna) was determined by protein chemistry techniques. The inhibitory specificity of alfaAI-1 was screened with a panel orthe diges- tive alfa-amylases from 30 species of insects, mites, gastropod, annelid worm, nematode and fungal phytopathogens with afrocus on agricultural pests and important model species. This in vitro analysis showed a selective inhibition of alfa-amylases from three orders or insect (Coleoptera, Hymenoptera and Diptera) and an inhibition of alfa-amylases of the annelid worm. The inhibitory potential of alfaAI-1 against several alfa-amylases was found to be modulated by pH. To understand how alfaAI-1discriminates among closely related alfa-amylases, the sequences of the alfa-amylases sensitive, respectively, insensitive to alfaAI-1 were compared, and the critical determinants were localized on the spatial alfa-amylase model. Based o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F04%2F1286" target="_blank" >GA522/04/1286: Insekticidní proteiny cílené proti trávicímu traktu hmyzu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phytochemistry

ISSN

0031-9422

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

31-39

Kód UT WoS článku

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EID výsledku v databázi Scopus

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