Inhibitory spec. a insektic. selektivity na ;-amylase inhibitor z rostlin Phaseolus vulgaris

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A8184" target="_blank" >RIV/60460709:41210/04:8184 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inhibitory specificity and insecticidal selectivity of α-amylase inhibitor from Phaseolus vulgaris.

Popis výsledku v původním jazyce

The primary structure and proteolytic processing of the &#945;-amylase isoinhibitor &#945; AI-1 from common bean (Phaseolus vulgaris cv. Magna) was determined by protein chemistry techniques. The inhibitory specificity of &#945;AI-1 was screened with a panel of the digestive &#945;-amylases from 30 species of insects, mites, gastropod, annelid worm, nematode and fungal phytopathogens with a focus on agricultural pests and important model species. This in vitro analysis showed a selective inhibition of &#945;-amylases from three orders of insect (Coleoptera, Hymenoptera and Diptera) and an inhibition of &#945;-amylases of the annelid worm. The inhibitory potential of &#945;AI-1 against several &#945;-amylases was found to be modulated by pH. To understand how &#945;AI-1 discriminates among closely related &#945;-amylases, the sequences of the &#945;-amylases sensitive, respectively, insensitive to &#945;AI-1 were compared, and the critical determinants were localized on the spatial &#94

Název v anglickém jazyce

Inhibitory specificity and insecticidal selectivity of α-amylase inhibitor from Phaseolus vulgaris.

Popis výsledku anglicky

The primary structure and proteolytic processing of the &#945;-amylase isoinhibitor &#945; AI-1 from common bean (Phaseolus vulgaris cv. Magna) was determined by protein chemistry techniques. The inhibitory specificity of &#945;AI-1 was screened with a panel of the digestive &#945;-amylases from 30 species of insects, mites, gastropod, annelid worm, nematode and fungal phytopathogens with a focus on agricultural pests and important model species. This in vitro analysis showed a selective inhibition of &#945;-amylases from three orders of insect (Coleoptera, Hymenoptera and Diptera) and an inhibition of &#945;-amylases of the annelid worm. The inhibitory potential of &#945;AI-1 against several &#945;-amylases was found to be modulated by pH. To understand how &#945;AI-1 discriminates among closely related &#945;-amylases, the sequences of the &#945;-amylases sensitive, respectively, insensitive to &#945;AI-1 were compared, and the critical determinants were localized on the spatial &#94

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phytochemistry

ISSN

0031-9422

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

31-39

Kód UT WoS článku

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EID výsledku v databázi Scopus

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