Kryoprotokoly používané pro kryoprezervaci vegetativně množených rostlin v České kryobance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A2193" target="_blank" >RIV/00027006:_____/07:2193 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank

Popis výsledku v původním jazyce

In the last decade, the safety of the collection of vegetatively propagated crops in the Czech Republic led also to the application of the cryopreservation technology. At present, the main vegetatively-propagated crops cryopreserved in the Czech cryobankinclude Allium spp., potato, apple, pear, Prunus spp., strawberry and hop. In apple, a ?two-step freezing? protocol and the encapsulation-dehydration method are used with dormant buds and shoot tips, respectively. The ?ultra-rapid cryopreservation method?, consisting in the quenching in liquid nitrogen of aluminium foils containing dehydrated shoot tips, is used for potato and hop meristems. As for Allium, best results are obtained after shoot tips, excised from bulblets and microbulbs, are treated with PVS3 (50% glycerol, 50% sucrose) and directly plunged into liquid nitrogen. The DSC (Differential Scanning Calorimetry) is an important tool to carry out basic themodynamic studies on the temperature of glass transition in meristematic

Název v anglickém jazyce

Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank

Popis výsledku anglicky

In the last decade, the safety of the collection of vegetatively propagated crops in the Czech Republic led also to the application of the cryopreservation technology. At present, the main vegetatively-propagated crops cryopreserved in the Czech cryobankinclude Allium spp., potato, apple, pear, Prunus spp., strawberry and hop. In apple, a ?two-step freezing? protocol and the encapsulation-dehydration method are used with dormant buds and shoot tips, respectively. The ?ultra-rapid cryopreservation method?, consisting in the quenching in liquid nitrogen of aluminium foils containing dehydrated shoot tips, is used for potato and hop meristems. As for Allium, best results are obtained after shoot tips, excised from bulblets and microbulbs, are treated with PVS3 (50% glycerol, 50% sucrose) and directly plunged into liquid nitrogen. The DSC (Differential Scanning Calorimetry) is an important tool to carry out basic themodynamic studies on the temperature of glass transition in meristematic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QF3039" target="_blank" >QF3039: Založení kryobanky pro konzervaci vegetativních vrcholů bramboru a chmele.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advances in horticultural science

ISSN

0394-6169

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

4

Strana od-do

247-250

Kód UT WoS článku

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EID výsledku v databázi Scopus

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