Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F10%3A00001138" target="_blank" >RIV/00027006:_____/10:00001138 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

Popis výsledku v původním jazyce

The appropriate application of quantitative real-time PCR (RT-qPCR), requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability. The expression of seven reference genes was evaluated in tissues of 180 healthy, physiologically stressed and virus-infected cereal plants by RT-qPCR. The expression of all genes did not depend on abiotic stress conditions or virus infections. GAPDH, TUBB and 18S rRNA alwaysranked as the three most stable genes. On the other hand, EF1A, EIF4A, and 28S rRNA for barley and oat samples; and TUBA for wheat samples were consistently ranked as the less reliable controls. The geometric average of GAPDH, 18S rRNA and TUBB is suitable for normalisation of BYDV quantification in barley tissues. For wheat and oat samples, a combination of four genes is necessary: GAPDH, 18SrRNA, TUBB and EIF4A for wheat; and GAPDH, 18S rRNA, TUBB and TUBA for oat is recommended.

Název v anglickém jazyce

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

Popis výsledku anglicky

The appropriate application of quantitative real-time PCR (RT-qPCR), requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability. The expression of seven reference genes was evaluated in tissues of 180 healthy, physiologically stressed and virus-infected cereal plants by RT-qPCR. The expression of all genes did not depend on abiotic stress conditions or virus infections. GAPDH, TUBB and 18S rRNA alwaysranked as the three most stable genes. On the other hand, EF1A, EIF4A, and 28S rRNA for barley and oat samples; and TUBA for wheat samples were consistently ranked as the less reliable controls. The geometric average of GAPDH, 18S rRNA and TUBB is suitable for normalisation of BYDV quantification in barley tissues. For wheat and oat samples, a combination of four genes is necessary: GAPDH, 18SrRNA, TUBB and EIF4A for wheat; and GAPDH, 18S rRNA, TUBB and TUBA for oat is recommended.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QH81269" target="_blank" >QH81269: Inovace diagnostických metod a ochranných opatření vůči virovým zakrslostem obilnin a jejich vektorům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Plant Biology

ISSN

1471-2229

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

léto

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

759

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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