Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00001757" target="_blank" >RIV/00027006:_____/11:00001757 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x" target="_blank" >10.1111/j.1439-0434.2011.01790.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

Popis výsledku v původním jazyce

SYBR Green real-time RT PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus(MbFV). The polyvalence of the designed primers was tested on 50 genotypes of garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97,93, 99, 98 and 87% for OYDV, LYSV, SLV, GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies and for MbFV it was 130 gene copies. In comparison with ELISA, morevirus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green based real-time RT PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses.

Název v anglickém jazyce

Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

Popis výsledku anglicky

SYBR Green real-time RT PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus(MbFV). The polyvalence of the designed primers was tested on 50 genotypes of garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97,93, 99, 98 and 87% for OYDV, LYSV, SLV, GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies and for MbFV it was 130 gene copies. In comparison with ELISA, morevirus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green based real-time RT PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH71228" target="_blank" >QH71228: Ozdravení domácích genotypů česneku za účelem jejich uchování metodou kryokonzervace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology

ISSN

0931-1785

e-ISSN

—

Svazek periodika

159

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

429-434

Kód UT WoS článku

000289626700006

EID výsledku v databázi Scopus

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