Proteomic analysis of plant response to drought with comparison of gel-based and gel-free proteomic approaches

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005647" target="_blank" >RIV/00027006:_____/19:00005647 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of plant response to drought with comparison of gel-based and gel-free proteomic approaches

Popis výsledku v původním jazyce

Proteomic analysis of plant response to drought with comparison of gel-based and gel-free proteomic approaches Tereza Bláhová 1,2 *, Pavel Vítámvás 2 , Jenny Renaut 3 , Radovan Hynek 1 1. Vysoká škola chemicko-technologická v Praze 2. Výzkumný ústav rostlinné výroby 3. Luxembourg Institute of Science and Technology In despite of the fact that drought is the most crop influencing factors in the world, a mechanism of drought tolerance in various plants is still unclear. The aim of our study lies in an observation of changes in wheat proteome under drought conditions and in a comparison of two proteomic approaches – gel based and gel free methods. For deeper clarification of wheat response to drought two tissues of two wheat varieties with differ in drought tolerance and were analysed. The samples were analysed by gel-based method 2D-DIGE and significant changed spots were identified by MALDI-TOF-MS/MS. Mass spectra with ambiguous identification were analysed manually by de novo sequencing. Altogether, 262 crown spots, resp. 259 leaf, were analysed, 168, resp. 192 of them were identified. By gel-free LC-MS/MS method were more than 1000 proteins in both tissues significant identified with at least 1 unique peptide or 2 peptides overall, but statistical analysis are still in process. Focusing on late embryogenesis abundant proteins (LEA), important protective proteins, showed interesting protein profiles in wheat varieties. By 2D-DIGE of crowns we found 1 LEA 3 with faster biosynthesis and 3 isoforms of LEA1 in tolerant variety compare to sensitive variety with slower response and only 2 isoforms of LEA 1. In leaves, except slower biosynthesis of 2 isoforms of LEA 1 in both varieties, any significant differences between varieties weren’t observed. Surprisingly, by LC-MS/MS were no LEA 3 neither LEA 1 found but founded 4 proteins from LEA 2 group (directly involved in plant response to drought) were differently expressed in high concentrations (some of them more in sensitive variety). Clarification of these results is interesting task of the following days and for future studies. In: 8th Annual Conference of the Czech Society for Mass Spectrometry, 27.3.-29.3.2019. Olomouc, Česká společnost pro hmotnostní spektrometrii, 2019. s. 50. ISBN 978-80-905045-9-2

Název v anglickém jazyce

Proteomic analysis of plant response to drought with comparison of gel-based and gel-free proteomic approaches

Popis výsledku anglicky

Proteomic analysis of plant response to drought with comparison of gel-based and gel-free proteomic approaches Tereza Bláhová 1,2 *, Pavel Vítámvás 2 , Jenny Renaut 3 , Radovan Hynek 1 1. Vysoká škola chemicko-technologická v Praze 2. Výzkumný ústav rostlinné výroby 3. Luxembourg Institute of Science and Technology In despite of the fact that drought is the most crop influencing factors in the world, a mechanism of drought tolerance in various plants is still unclear. The aim of our study lies in an observation of changes in wheat proteome under drought conditions and in a comparison of two proteomic approaches – gel based and gel free methods. For deeper clarification of wheat response to drought two tissues of two wheat varieties with differ in drought tolerance and were analysed. The samples were analysed by gel-based method 2D-DIGE and significant changed spots were identified by MALDI-TOF-MS/MS. Mass spectra with ambiguous identification were analysed manually by de novo sequencing. Altogether, 262 crown spots, resp. 259 leaf, were analysed, 168, resp. 192 of them were identified. By gel-free LC-MS/MS method were more than 1000 proteins in both tissues significant identified with at least 1 unique peptide or 2 peptides overall, but statistical analysis are still in process. Focusing on late embryogenesis abundant proteins (LEA), important protective proteins, showed interesting protein profiles in wheat varieties. By 2D-DIGE of crowns we found 1 LEA 3 with faster biosynthesis and 3 isoforms of LEA1 in tolerant variety compare to sensitive variety with slower response and only 2 isoforms of LEA 1. In leaves, except slower biosynthesis of 2 isoforms of LEA 1 in both varieties, any significant differences between varieties weren’t observed. Surprisingly, by LC-MS/MS were no LEA 3 neither LEA 1 found but founded 4 proteins from LEA 2 group (directly involved in plant response to drought) were differently expressed in high concentrations (some of them more in sensitive variety). Clarification of these results is interesting task of the following days and for future studies. In: 8th Annual Conference of the Czech Society for Mass Spectrometry, 27.3.-29.3.2019. Olomouc, Česká společnost pro hmotnostní spektrometrii, 2019. s. 50. ISBN 978-80-905045-9-2

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/QK1710302" target="_blank" >QK1710302: Zvýšení odolnosti pšenic vůči suchu, mrazu, padlí a fuzariózám klasu pomocí metod genomiky a proteomiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů