Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149485" target="_blank" >RIV/00027006:_____/20:10149485 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00020702:_____/20:N0000062

Výsledek na webu

<a href="https://bp.ueb.cas.cz/pdfs/bpl/2020/01/28.pdf" target="_blank" >https://bp.ueb.cas.cz/pdfs/bpl/2020/01/28.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.32615/bp.2019.148" target="_blank" >10.32615/bp.2019.148</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservation

Popis výsledku v původním jazyce

Cryostorage techniques have been developed to preserve the most valuable genotype from an endangered native population of Populus x canescens Aiton Sm. (grey poplar), which is located in the floodplain forest in the South Moravia region of the Czech Republic and which is difficult to propagate using cuttings. The prevailing genotype with valuable traits was selected by the simple sequence repeats method. This genotype was used to determine the most effective pre-cultivation conditions (cold hardening, and cold hardening combine with osmotic treatment) on dehydration tolerance and post-thaw recovery of the grey poplar shoot tips. The pre-cultivation and application of a modified plant vitrification solution 3 (PVS3) considerable reduced the freezable water content in shoot tips. Evaluation after eight weeks of regrowth revealed that simultaneous effect of cold and osmotic pre-treatments and application of PVS3 for 120 min enabled 93.3 +/- 5.8 % recovery. The results emphasize the importance of the pre-cultivation conditions, which significantly improved the post-thaw recovery of the grey poplar explants.

Název v anglickém jazyce

Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservation

Popis výsledku anglicky

Cryostorage techniques have been developed to preserve the most valuable genotype from an endangered native population of Populus x canescens Aiton Sm. (grey poplar), which is located in the floodplain forest in the South Moravia region of the Czech Republic and which is difficult to propagate using cuttings. The prevailing genotype with valuable traits was selected by the simple sequence repeats method. This genotype was used to determine the most effective pre-cultivation conditions (cold hardening, and cold hardening combine with osmotic treatment) on dehydration tolerance and post-thaw recovery of the grey poplar shoot tips. The pre-cultivation and application of a modified plant vitrification solution 3 (PVS3) considerable reduced the freezable water content in shoot tips. Evaluation after eight weeks of regrowth revealed that simultaneous effect of cold and osmotic pre-treatments and application of PVS3 for 120 min enabled 93.3 +/- 5.8 % recovery. The results emphasize the importance of the pre-cultivation conditions, which significantly improved the post-thaw recovery of the grey poplar explants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/QK1910277" target="_blank" >QK1910277: Využití metody kryoprezervace pro zefektivnění šlechtitelského procesu hospodářsky významných zemědělských plodin a uchování lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOLOGIA PLANTARUM

ISSN

0006-3134

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

2020

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

119-128

Kód UT WoS článku

000514618400001

EID výsledku v databázi Scopus

2-s2.0-85084065944