Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177161" target="_blank" >RIV/00027006:_____/24:10177161 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/14864347:_____/24:N0000010

Výsledek na webu

<a href="https://www.mdpi.com/2223-7747/13/11/1578/pdf?version=1718178784" target="_blank" >https://www.mdpi.com/2223-7747/13/11/1578/pdf?version=1718178784</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants13111578" target="_blank" >10.3390/plants13111578</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding

Popis výsledku v původním jazyce

This study investigated the thermal properties of potato and hop pollen for cryopreservation and subsequent cross-breeding. Phase transitions and frozen water content in selected pollen samples were measured using a differential scanning calorimeter (DSC). Unlike hop pollen, potato pollen showed high variability in thermal properties and water content. Three specific types of pollen samples based on their thermal characteristics and water content were distinguished by DSC in potato: (1) &apos;glassy&apos;, with a water content lower than 0.21 g water per g dry matter; (2) &apos;transient&apos;, with a water content between 0.27 and 0.34 g of water per g of dry matter; (3) &apos;frozen&apos;, with a water content higher than 0.34 g of water per g of dry matter. Only the &apos;glassy&apos; pollen samples with a low water content showed suitable properties for its long-term storage using cryopreservation in potato and hops. Cryopreservation of pollen did not significantly reduce its viability, and cryopreserved pollen was successfully used to produce both potato and hop hybrids. The results indicate that cryopreservation is a feasible technique for the preservation and utilization of pollen of these crops in the breeding process.

Název v anglickém jazyce

Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding

Popis výsledku anglicky

This study investigated the thermal properties of potato and hop pollen for cryopreservation and subsequent cross-breeding. Phase transitions and frozen water content in selected pollen samples were measured using a differential scanning calorimeter (DSC). Unlike hop pollen, potato pollen showed high variability in thermal properties and water content. Three specific types of pollen samples based on their thermal characteristics and water content were distinguished by DSC in potato: (1) &apos;glassy&apos;, with a water content lower than 0.21 g water per g dry matter; (2) &apos;transient&apos;, with a water content between 0.27 and 0.34 g of water per g of dry matter; (3) &apos;frozen&apos;, with a water content higher than 0.34 g of water per g of dry matter. Only the &apos;glassy&apos; pollen samples with a low water content showed suitable properties for its long-term storage using cryopreservation in potato and hops. Cryopreservation of pollen did not significantly reduce its viability, and cryopreserved pollen was successfully used to produce both potato and hop hybrids. The results indicate that cryopreservation is a feasible technique for the preservation and utilization of pollen of these crops in the breeding process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910277" target="_blank" >QK1910277: Využití metody kryoprezervace pro zefektivnění šlechtitelského procesu hospodářsky významných zemědělských plodin a uchování lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants-Basel

ISSN

2223-7747

e-ISSN

2223-7747

Svazek periodika

13

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

1578

Kód UT WoS článku

001247016700001

EID výsledku v databázi Scopus

2-s2.0-85195869770