Development and use of the cryopreservation method for the preservation of plant genetic resources and breeding material

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F23%3AN0000077" target="_blank" >RIV/14864347:_____/23:N0000077 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/23:10176628

Výsledek na webu

<a href="https://www.ishs.org/symposium/590" target="_blank" >https://www.ishs.org/symposium/590</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development and use of the cryopreservation method for the preservation of plant genetic resources and breeding material

Popis výsledku v původním jazyce

FALTUS, Milos, Jaroslava DOMKAROVA, Petr SVOBODA, Vendulka HORACKOVA, Vladimir NESVADBA, Olena BOBROVA, Stacy HAMMOND, Alois BILAVCIK and Jiri ZAMECNIK. Development and use of the cryopreservation method for the preservation of plant genetic resources and breeding material. In: Book of Abstracts from The IV International Symposium on Plant Cryopreservation. International Society for Horticultural Science, 12 - 15 June 2023, Oslo, Norway. 2023. p. 17. The Czech Crop Cryobank stored in total more than 500 accessions of 14 different crop species. The aim of this work was to determine the effect of dehydration on the pollen and explant thermal properties and their vitality in potatoes and hops. Explant survival and regeneration ability after cryopreservation was affected by genotype, significantly increased by osmotic stress, and depended on dehydration using the modified cryoprotective solution PVS3. The optimal explant dehydration time (60 minutes) led to a significant decrease in free water content to 0.3–5 %, while further dehydration led to a decrease in their vitality. When pollen water content failed below 0.27 g of water per g of dry matter, water does not crystallize and at the same time, the presence of a glass transition has always been demonstrated. With strong dehydration of pollen to 0.1 g of water per g of dry matter, the glass transition temperature increased to -5 °C, which represents increase in the safe storage temperature of pollen as well. Results showed that a suitable method of dehydration can limit the proportion of frozen water in explants in tested species of plants and the observed thermal properties of pollen confirm the suitability of the cryopreservation method for their long-term storage in liquid nitrogen conditions.

Název v anglickém jazyce

Development and use of the cryopreservation method for the preservation of plant genetic resources and breeding material

Popis výsledku anglicky

FALTUS, Milos, Jaroslava DOMKAROVA, Petr SVOBODA, Vendulka HORACKOVA, Vladimir NESVADBA, Olena BOBROVA, Stacy HAMMOND, Alois BILAVCIK and Jiri ZAMECNIK. Development and use of the cryopreservation method for the preservation of plant genetic resources and breeding material. In: Book of Abstracts from The IV International Symposium on Plant Cryopreservation. International Society for Horticultural Science, 12 - 15 June 2023, Oslo, Norway. 2023. p. 17. The Czech Crop Cryobank stored in total more than 500 accessions of 14 different crop species. The aim of this work was to determine the effect of dehydration on the pollen and explant thermal properties and their vitality in potatoes and hops. Explant survival and regeneration ability after cryopreservation was affected by genotype, significantly increased by osmotic stress, and depended on dehydration using the modified cryoprotective solution PVS3. The optimal explant dehydration time (60 minutes) led to a significant decrease in free water content to 0.3–5 %, while further dehydration led to a decrease in their vitality. When pollen water content failed below 0.27 g of water per g of dry matter, water does not crystallize and at the same time, the presence of a glass transition has always been demonstrated. With strong dehydration of pollen to 0.1 g of water per g of dry matter, the glass transition temperature increased to -5 °C, which represents increase in the safe storage temperature of pollen as well. Results showed that a suitable method of dehydration can limit the proportion of frozen water in explants in tested species of plants and the observed thermal properties of pollen confirm the suitability of the cryopreservation method for their long-term storage in liquid nitrogen conditions.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910277" target="_blank" >QK1910277: Využití metody kryoprezervace pro zefektivnění šlechtitelského procesu hospodářsky významných zemědělských plodin a uchování lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů