Effect of Sucrose Concentration on the in Vitro Survival Rate of Garlic from the Basal Plate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179571" target="_blank" >RIV/00027006:_____/25:10179571 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Sucrose Concentration on the in Vitro Survival Rate of Garlic from the Basal Plate

Popis výsledku v původním jazyce

This study aimed to determine the effect of different sucrose concentrations added in the culture medium on the growth of the garlic (Allium sativum L.) basal plate under in vitro conditions. Sucrose provides carbon and energy for plant metabolism and affects osmotic balance and tissue physiology. Therefore, optimizing its concentration is crucial for successful growth and regeneration of plant material in controlled laboratory environments.In this experiment, sucrose was added to the culture medium at concentrations of 0, 5, 10, 15, 30, 60, and 120 g·l⁻¹. Basal plate explants were cultivated under standard in vitro conditions, and after the cultivation period, their morphological response, survival, regeneration, and vitality were evaluated. The results confirmed that sucrose concentration significantly affected growth and physiological condition of the tissues. The best results were obtained at 10, 15 and 30 g·l⁻¹, where explants exhibited the most vigorous growth, healthy coloration, and high vitality. In contrast, the variant without sucrose showed the poorest growth and the lowest vitality of the explants.The findings contribute to a better understanding of the role of sucrose in garlic in vitro culture and can be applied in the optimization of subsequent biotechnological procedures, such as the preparation of plant material for cryopreservation. Determining the optimal sucrose concentration is therefore an important step toward maintaining tissue vitality and ensuring efficient plant regeneration after demanding preservation processes. Prezentace posteru na konferenci Kostelecké inspirování 27.11.2025

Název v anglickém jazyce

Effect of Sucrose Concentration on the in Vitro Survival Rate of Garlic from the Basal Plate

Popis výsledku anglicky

This study aimed to determine the effect of different sucrose concentrations added in the culture medium on the growth of the garlic (Allium sativum L.) basal plate under in vitro conditions. Sucrose provides carbon and energy for plant metabolism and affects osmotic balance and tissue physiology. Therefore, optimizing its concentration is crucial for successful growth and regeneration of plant material in controlled laboratory environments.In this experiment, sucrose was added to the culture medium at concentrations of 0, 5, 10, 15, 30, 60, and 120 g·l⁻¹. Basal plate explants were cultivated under standard in vitro conditions, and after the cultivation period, their morphological response, survival, regeneration, and vitality were evaluated. The results confirmed that sucrose concentration significantly affected growth and physiological condition of the tissues. The best results were obtained at 10, 15 and 30 g·l⁻¹, where explants exhibited the most vigorous growth, healthy coloration, and high vitality. In contrast, the variant without sucrose showed the poorest growth and the lowest vitality of the explants.The findings contribute to a better understanding of the role of sucrose in garlic in vitro culture and can be applied in the optimization of subsequent biotechnological procedures, such as the preparation of plant material for cryopreservation. Determining the optimal sucrose concentration is therefore an important step toward maintaining tissue vitality and ensuring efficient plant regeneration after demanding preservation processes. Prezentace posteru na konferenci Kostelecké inspirování 27.11.2025

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů