Evaluation of insemination doses from boars by flow cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F22%3AN0000271" target="_blank" >RIV/00027014:_____/22:N0000271 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.respigbreed.cz/2022/1/1.pdf" target="_blank" >http://www.respigbreed.cz/2022/1/1.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of insemination doses from boars by flow cytometry

Popis výsledku v původním jazyce

The objective of this study was to assessed the sperm viability and acrosome integrity in boar insemination doses (ID) prepared in short-term and long-term extender by flow cytometry. Fifty-eight ejaculates from 6 healthy and fertile AI boars were used for this study. Fresh boar semen was diluted in long-term commercial extenders Androhep (A), Androstar plus (AS+) and short-term extender VIP 5 in a semen-dilution ratio of 1+2. ID were stored at a 17°C. Sperm viability, acrosome integrity and progressive sperm motility were evaluated in ID after 24h and 48h storage time. According results, there were not found differences between extenders during storage time in ID in sperm viability, acrosome integrity and progressive motility (p>0.05). In conclusion, the present study did not find a significant difference between long and short-term extender in progressive sperm motility, viability and acrosome integrity in ID for 24 h to 48 h of storage time.

Název v anglickém jazyce

Evaluation of insemination doses from boars by flow cytometry

Popis výsledku anglicky

The objective of this study was to assessed the sperm viability and acrosome integrity in boar insemination doses (ID) prepared in short-term and long-term extender by flow cytometry. Fifty-eight ejaculates from 6 healthy and fertile AI boars were used for this study. Fresh boar semen was diluted in long-term commercial extenders Androhep (A), Androstar plus (AS+) and short-term extender VIP 5 in a semen-dilution ratio of 1+2. ID were stored at a 17°C. Sperm viability, acrosome integrity and progressive sperm motility were evaluated in ID after 24h and 48h storage time. According results, there were not found differences between extenders during storage time in ID in sperm viability, acrosome integrity and progressive motility (p>0.05). In conclusion, the present study did not find a significant difference between long and short-term extender in progressive sperm motility, viability and acrosome integrity in ID for 24 h to 48 h of storage time.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40203 - Husbandry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

1-4

Kód UT WoS článku

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EID výsledku v databázi Scopus

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