Asessment of Boar Sperm Viability Using by Flow Cytometry and by Eosin-Nigrosin Staining
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F23%3A10005761" target="_blank" >RIV/00027014:_____/23:10005761 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.respigbreed.cz/2023/1/1.pdf" target="_blank" >https://www.respigbreed.cz/2023/1/1.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Asessment of Boar Sperm Viability Using by Flow Cytometry and by Eosin-Nigrosin Staining
Popis výsledku v původním jazyce
The objective of this study was to assessed the percentage of viable spermatozoa of boar using by flow cytometry and by eosin-nigrosine staining. Fifteen fertile Přeštice black-pied boars of various ages (1- 5 years) coming from one AI centre were used in this study. Ejaculates (n=169) were collected using the gloved-hand technique during 3 years. The basic parameters of boar sperm quality were assessment and percentage of sperm viable spermatozoa were determined by flow cytometry using SYBR-14 and propidium iodide and by a supravital staining technique using the eosin-nigrosine stain mixture. According results, there is no statistically significant difference between the methods of determining the percentage of viable spermatozoa (p=0.95). A statistically significant correlation coefficient was found between the methods (p>0.05). In conclusion, on the basis of the results, it was found that both methods of determining viable spermatozoa enable to compare the integrity of boar spermatozoa membranes and thus to assess the quality of insemination doses.
Název v anglickém jazyce
Asessment of Boar Sperm Viability Using by Flow Cytometry and by Eosin-Nigrosin Staining
Popis výsledku anglicky
The objective of this study was to assessed the percentage of viable spermatozoa of boar using by flow cytometry and by eosin-nigrosine staining. Fifteen fertile Přeštice black-pied boars of various ages (1- 5 years) coming from one AI centre were used in this study. Ejaculates (n=169) were collected using the gloved-hand technique during 3 years. The basic parameters of boar sperm quality were assessment and percentage of sperm viable spermatozoa were determined by flow cytometry using SYBR-14 and propidium iodide and by a supravital staining technique using the eosin-nigrosine stain mixture. According results, there is no statistically significant difference between the methods of determining the percentage of viable spermatozoa (p=0.95). A statistically significant correlation coefficient was found between the methods (p>0.05). In conclusion, on the basis of the results, it was found that both methods of determining viable spermatozoa enable to compare the integrity of boar spermatozoa membranes and thus to assess the quality of insemination doses.
Klasifikace
Druh
J<sub>ost</sub> - Ostatní články v recenzovaných periodicích
CEP obor
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OECD FORD obor
40203 - Husbandry
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Research in Pig Breeding
ISSN
1802-7547
e-ISSN
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Svazek periodika
17
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
3
Strana od-do
1-3
Kód UT WoS článku
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EID výsledku v databázi Scopus
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