Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73832" target="_blank" >RIV/60460709:41210/17:73832 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.anireprosci.2017.07.020" target="_blank" >http://dx.doi.org/10.1016/j.anireprosci.2017.07.020</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2017.07.020" target="_blank" >10.1016/j.anireprosci.2017.07.020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm

Popis výsledku v původním jazyce

An intact and functional sperm plasmalemma has a major role in sperm motility and fertilizing capacity. Several techniques have been developed to evaluate the integrity of the sperm plasma membrane, but there are still some inconsistencies concerning the methods that are more closely associated with sperm function. In this study, the aim was to: i) evaluate the integrity of the boar sperm plasmalemma during 72 h of semen storage at 17C using four techniques: eosin/nigrosin (E/N), propidium iodide/carboxyfluorescein diacetate (PI/CFDA), hypo-osmotic swelling test (HOST), and combined HOST with eosin staining (HOST/E), ii) assess the correlations and the limits of consistency among these techniques, iii) and estimate the relationships with the acrosomal status and sperm kinetics. Results indicate that the integrity of the sperm plasmalemma decreases during 72 h of storage, although significant differences were found only using the HOST and HOST/E techniques. Moreover, use of E/N and PI/CFDA results in

Název v anglickém jazyce

Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm

Popis výsledku anglicky

An intact and functional sperm plasmalemma has a major role in sperm motility and fertilizing capacity. Several techniques have been developed to evaluate the integrity of the sperm plasma membrane, but there are still some inconsistencies concerning the methods that are more closely associated with sperm function. In this study, the aim was to: i) evaluate the integrity of the boar sperm plasmalemma during 72 h of semen storage at 17C using four techniques: eosin/nigrosin (E/N), propidium iodide/carboxyfluorescein diacetate (PI/CFDA), hypo-osmotic swelling test (HOST), and combined HOST with eosin staining (HOST/E), ii) assess the correlations and the limits of consistency among these techniques, iii) and estimate the relationships with the acrosomal status and sperm kinetics. Results indicate that the integrity of the sperm plasmalemma decreases during 72 h of storage, although significant differences were found only using the HOST and HOST/E techniques. Moreover, use of E/N and PI/CFDA results in

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Svazek periodika

184

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

218-227

Kód UT WoS článku

000411300700025

EID výsledku v databázi Scopus

2-s2.0-85026665960